|Table of Contents|

Cultivation of cry 1Iem Gene Transformed Soybean and Insect Resistant Assay(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2009年06期
Page:
959-963
Research Field:
Publishing date:

Info

Title:
Cultivation of cry 1Iem Gene Transformed Soybean and Insect Resistant Assay
Author(s):
CHEN Xiu-hua; BAI Xi; PAN Xin; ZHAI Hong; CAI Hua; JI Wei; LI Yong; ZHU Yan-ming
Plant Bioengineering Laboratory，College of Life Sciences，Northeast Agriculture University，Harbin 150030，Heilongjiang，China
Keywords:
Soybean; cry1Iem gene; Contyledonary nodes; Transformed resistant plants; Insect resistant assay
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.06.959
Abstract:
Contyledonary nodes were used as explants，and insect resistant gene cry1Iem was transformed into soybean via Agrobacterium-mediated method.Bar served as selection marker，and glufasinate was used in explants transformation to screen resistant shoots.Lots of resistant plants were obtained.With PCR detection of T0、T1、T2 generation，insect resistant gene cry1Iem was proved to be transformed into soybean preliminarily.Furthermore，immature pods of PCR positive plants in T2 generation and newly hatched larvaes were introduced，and initial insect resistant assay was made by disk method.Result suggested that one transgenic plant had an obvious resistant effect，and another seven plants proved to have some effect.

References:

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Last Update: 2014-09-11