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Glyphosate Resistant Mutant Strain of Soybean Filtered by Chemomorphosis(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2009年05期
Page:
938-940
Research Field:
Publishing date:

Info

Title:
Glyphosate Resistant Mutant Strain of Soybean Filtered by Chemomorphosis
Author(s):
ZHANG Li-li1; GU Wei2; LEI Bo-jun1; LU Xiao-bo1; LI Tie1
1.Biotechnology Research Institute，Heilongjiang Academy of Agricultural Sciences，Harbin 150086；
?2.Crop Nutrition Practical Technology Institute of Heilongjiang Academy of Agricultural Sciences，Harbin 150086，Heilongjiang，China
Keywords:
Soybean; Ethyl methane sulphonate; Sodium azide; Chemomorphosis; Glyphosate resistance
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.05.0938
Abstract:
To screen soybean lines resistant to glyphosate through chemomorphosis，six soybean varieties widly planted in Heilongjiang Province were dealt with chemical mutagen ethyl methane sulphonate（EMS）and sodium azide by seed soaking.The survival rate of soybean seeds in M1generation was higher than that of semi-lethal concentrations.In M3generation，35518 plants were identified by spraying 1.31ai.kg.ha-1glyphosate at three-leaf stage，from which a total of 40 resistant plants showed normal growth，and they were Suinong 10 and Heinong 44 dealt with 5 mmol.L-1 sodium azide.The M4generation of the 40 survival lines also showed glyphosate resistance.Results suggest chemical mutagen could be used in selecting soybean mutant lines resistant to glyphosate.

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Last Update: 2014-09-19