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Study on Immobilized β-D-fructofuranosidase by Cross-linking with Glutaraldehyde(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2009年05期
Page:
902-905
Research Field:
Publishing date:

Info

Title:
Study on Immobilized β-D-fructofuranosidase by Cross-linking with Glutaraldehyde
Author(s):
YANG Xiu-fang; CHEN Mei
Key Laboratory of Auxiliary Chemistry & Technology for Chemical Industry，Ministry of Education;Shaanxi University of Science & Technology，Xi’an 710021，Shaanxi，China
Keywords:
Cross-linking with glutaraldehyde; β-D-fructofuranosidase; Immobilization ennyme
PACS:
TS245.9
DOI:
10.11861/j.issn.1000-9841.2009.05.0902
Abstract:
The soybean oligosaccharide is a kind of functional oligosaccharides,which can multiply the number of Bifidobacteria.A novel method to immobilize β-D-fructofuranosidase by cross-linking with glutaraldehyde was successfully constructed.The preparation condition and operation stability of immobilization enzyme were investigated during the process of soybean oligosaccharide production.A novel method to immobilize β-D-fructofuranosidase by cross-linking with glutaraldehyde was successfully constructed.The conditions of immobilization were investigated by orthogonal experiment method.The optimal conditions of immobilization investigated by orthogonal experiment method were as follows：the concentration of glutaraldehyde and protein were 20 mmol.L-1and 1.0%，the ratio of enzyme and immobilization liquld was 1∶10，the immobilizing time was 2 h.Under the optimal condition the average activity of immobilized enzyme reached 340 U.g -1，after immobilization and the activity could retain more than 80%.The immobilized enzyme showed high operation stability in batch reactor，whose half-life was 58 d.The stability of immobilized enzyme could completely meet the need of industrial production.

References:

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Last Update: 2014-09-18