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Relationship Between Induced β-1，3-Glucanase and Resistance of Soybean to Phytophthora Root Rot(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2009年05期
Page:
883-888
Research Field:
Publishing date:

Info

Title:
Relationship Between Induced β-1，3-Glucanase and Resistance of Soybean to Phytophthora Root Rot
Author(s):
ZUO Yu-hu1; 2; YANG Chuan-ping1; ZHENG Yao3; LOU Shu-bao2
1. Postdoctoral Research Station of Forestry，Northeast Forestry University，Harbin 150040，Heilongjiang；
2.College of Agronomy，Heilongjiang Bayi Agricultural University，Daqing 163319，Heilongjiang；
3.Dalian Jinzhou Popularizing Agricultural Technique Center，Dalian 116100，Liaoning,China
Keywords:
Soybean; Phythophthora sojae; β-1; 3-glucanase; Disease resistance; Antifungal activity
PACS:
S435.651
DOI:
10.11861/j.issn.1000-9841.2009.05.0883
Abstract:
To know the relationship between induced β-1，3-glucanase and resistance of soybean to phytophthora root rot，the activities of β-1，3-glucanase in true leaves and first compound leaves of three soybean cultivars with different resistance after infection by Phythophthora sojae were detected.After infecting true leaves and first compound leaves，the activities of β-1，3-glucanase were enhanced in all cultivars.The enzyme-activity in true leaves of resistance cultivar reached the peak at 48 hours after inoculation，increasing 105.9%，and middle susceptible and susceptible cultivars reached the peck at 72 hours after inoculation，increasing 66.3% and 65.7%,respectively.The results showed the relationship between the activities of β-1，3-glucanase and the resistance of soybean cultivars were positive.The increase peaks of enzyme-activity changes appeared earlier in the resistant cultivars than the susceptible ones，and showed a higher level of enzyme-activity changes in the resistant cultivars.Still，the results of the partial enzyme properties indicated that the optimal temperature and pH of the β-1，3-glucanase was 55℃ and 5.0，respectively.The enzyme was stable at pH 4.5-7.5 and temperature below 55℃.The activities of β-1，3-glucanase were stimulated by Ca2+，Mn2+，K+，Al3+and inhibited by Mg2+，Cu2+，Zn2+，Fe3+，Fe2+，Hg2+，Ba2+.Antifungal activities showed that the mycelia growth and zoospore germination of P.sojae were significantly inhibited by crude enzyme.

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Last Update: 2014-09-18