|Table of Contents|

Screening on Soybean Resistance to Phytophthora sojae using Cotyledon Inoculation Method(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2009年05期
Page:
879-882，888
Research Field:
Publishing date:

Info

Title:
Screening on Soybean Resistance to Phytophthora sojae using Cotyledon Inoculation Method
Author(s):
YU An-liang1; XU Peng-fei1; CHEN Chen1; WANG Jin-sheng1; WU Jun-jiang2; LI Ning-hui1; LI Wen-bin1; MA Feng-ming3; QIU Li-juan4; CHANG Ru-zhen4; CHEN Wei-yuan5; ZHANG Shu-zhen1
1.Soybean Research Institute of Northeast Agricultural University，Key Laboratory of Soybean Biology of Chinese Education Ministry，Harbin 150030，Heilongjiang；2.Soybean Research Institute，Heilongjiang Academy of Agricultural Sciences，Harbin 150086，Heilongjiang；3.College of Agriculture，Northeast Agricultural University，Harbin 150030，Heilongjiang；
Keywords:
4.Crop Sciences Institute; Chinese Academy of Agricultural Sciences; Beijing 100081; Disease resistance identification; Cotyledon inoculation; Hypocotyl inoculation; Phytophthora sojae

PACS:
S435.651
DOI:
10.11861/j.issn.1000-9841.2009.05.0879
Abstract:
Soybean production is seriously affected by Phytophthora sojae，one kind of destructive fungal diseases.The most effective measure to prevent and control the diseases is to carry out disease-resistant breeding. In this paper，65 soybean varieties from Heilongjiang，Inner Mongolia，Hunan and Sichuan were evaluated the reactions to P.sojae race 1 by way of cotyledon inoculation.Of all the varieties tested，11 were resistant，49 were susceptible，and the rest were intermediate. Fifty-one varieties had the same reaction as those evaluated with hypocotyl inoculation method.There was significant correlation between cotyledon inoculation and hypocotyl inoculation method according to DPS V7.05 analysis.By the independent tests of r×c table of cotyledon inoculation and vaccination hypocotyl，there were no significant differences between the two methods.Cotyledon inoculation was proved to be feasible to evaluate soybean germplasm infection with P.sojae compared with the commonly used hypocotyl inoculation method.It not only provided a method to identify the soybean resistance to P.sojae，but also solved the problem that the seeds of susceptible plants generated from crossing progeny could not be kept using hypocotyl inoculation method.

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Last Update: 2014-09-18