|Table of Contents|

Efficacy and Mechanism of Biological Seed Coating Agent Against Soybean Root Rot Disease Caused by Fusarium oxysporum f.sp.vedolens(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2009年05期
Page:
863-868,874
Research Field:
Publishing date:

Info

Title:
Efficacy and Mechanism of Biological Seed Coating Agent Against Soybean Root Rot Disease Caused by Fusarium oxysporum f.sp.vedolens
Author(s):
ZHANG Shu-mei; WANG Yu-xia; ZHAO Xiao-yu; ZHANG Xian-cheng; MENG Li-qiang; LI Jing
Institute of Microbiology of Heilongjiang Academy of Science，Harbin 150010，Heilongjiang，China
Keywords:
Soybean; Biological seed coating agent; F.oxysporum f.sp.vedolens; Mechanism of antifungi
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.05.0863
Abstract:
The soybean root rot is a kind of main fungal disease in Heilongjiang province.It was mainly caused by F.oxysporum f.sp.Vedolens.Bacillus subtilis B29 could inhibit the growth of F.oxysporum f.sp.vedolens.In this study，we researched the efficacy and mechanism of biological seed coating agent against soybean root rot disease caused by F.oxysporumf.sp.vedolens by the method of mycelia growth，dual culture，spore germination，microscope inspection，field trail and PCR-DGGE.The results indicated that biological seed coating agent could inhibit the growth of F.oxysporum f.sp.vedolens， distort the mycelia and spore and enhance the enzyme activities of POD，PPO and PAL in soybean root.The biocontrol bacteria could reside in rhizosphere of soybean and did not effect on the population of soil microbes.The efficacy of seed coating agent against soybean root rot disease was over 60% in field trail.

References:

[1]付春旭.大豆根腐病的发生及综合防治[J].大豆通报，2006，5:13-15.（Fu C X.Occurrence of soybean root rot disease and its comprehensive control strategy[J].Soybean Bulletin，2006，5:13-15.)

[2]王士强，洪音，王伟利，等.不同生物制剂对大豆根腐病的防效及生理机制的研究[J].黑龙江八一农垦大学学报，2008，20(1)：20-23.（Wang S Q，Hong Y，Wang W L，et al.Study of difference biological reagents on the antagonism of soybean root rot and the physiological mechanism[J].Journal of Heilongjiang August First Land Reclamation University，2008，20(1)：20-23.)
[3]李宝英，马淑梅.大豆根腐病病原菌种类及抗原筛选[J].植物保护学报，2000，27(1)：91-92.（Li B Y，Ma S M.Pathogens of soybean root rot and screening of resistant sources[J].Journal of Plant Protection，2000，27(1)：91-92.)
[4]韩庆新，辛惠普.大豆根腐病主要病原菌对大豆幼苗致病性的初步研究[J].大豆科学，1990，9(2)：157-162.（Han Q X，Xin H P.A preliminary study on the pathogenicity of the main pathogens causing soybean seedling root rot[J].Soybean Science，1990，9(2):157-162.)
[5]侯德安.大豆根腐病重发原因及防治对策[J].种子世界，2004，5：45.（Hou D A.Occurrence of soybean root rot disease and its control strategy[J].Seed World，2004，5：45.)
[6] Cook R J，Bruckart W L，Coulson J R.Safety of microorganisms intended for pest and plant disease control：a framework for scientific evaluation[J].Biological Control，1996，7：333-351.
[7] Emmert A B E，Handelsman J.Biocontrol of plant disease：a(Gram-)positive perspective[J].FEMS Microbiology Letters，1999，171(1）：1-9.
[8] Khan M R，Fischer S，Egan D.Biological control of Fusarium seeding blight disease of wheat and barley[J].Phytopathology，2006，96(4）：386-394.
[9] Brian B，Gardener M.Biological control of plant pathogens:Research，commercialization，and application in the USA.APSnet feature［M］.Biological Control of Plant Pathogens,2002.
[10] Ei-Tarabily K A，Soliman M H，Nassar A H.Biological control of Sclerotinai minor using a chitinolytic bacterium and actinomycetes[J].Plnat Pathology，2000，49 ：573-583.
[11]郭荣君，刘杏忠，杨怀文，等.芽孢杆菌BH1防治大豆根腐病的效果及机制[J].中国生物防治，2003，19（4）：180-184.（Guo R J，Liu X Z，Yang H W，et al.Mechanism of rhizobacteria BH1(Bacillus spp.)to suppress soybean root rot disease caused by Fusarium spp[J].Chinese Journal of Biological Control，2003，19(4)：180-184.)
[12]穆常青，潘玮，陆庆光，等.枯草芽孢杆菌对稻瘟病的防治效果评价及机制初探[J].中国生物防治，2006，22（2）：158-160.（Mu C Q，Pan w，Lu Q G，et al.Inhibitory action Bacillus subtilisstrain B-322 against Magnaprothe grisea[J].Chinese Journal of Biological Control，2006，22(2):158-160.)
[13]李德全，陈志谊，聂涯锋.生防菌BS-916及突变菌株抗菌物质及其对水稻抗性诱导作用的研究[J].植物病理学报，2008，38(2)：192-198.（Li D Q，Chen Z Y，Nie Y F.Antifungal substances produced by a high-yielding mutant of Bs-916 and their effects inducing resistance on rice plant[J].ACTA Phytopathologica Sinica，2008，38(2)：192-198.)
[14]刘邮洲，陈志谊，刘永峰，等.拮抗菌株C8-8控病促生作用机制初探[J].中国生物防治，2007，23（4）：397-400.(Liu Y Z，Chen Z Y，Liu Y F，et al.Mechanisms of diseases control and growth promotion with biocontrol strain C8-8[J].Chinease Journal of Biological Control，2007，23(4)：397-400.)
[15]Zhou J，Mary B.James M T.DNA recovery from soils of diverse composition[J].Applied Environmental Microbiology，1992，62：316-322.
[16]Muyzer G，Ellen C W，Andre G U.Profiling of complex microbial populations by denaturing gradient gel electrophores is analysis of polymerase chain reaction genes coding for 16S rRNA[J].Applied Environmental Mocrobiology,1993，59：695-700.
[17] Zwart G，Huismans R，Agterveld M P.Divergent members of the bacterial division-Verrucomicrobiales-in a temperate freshwater lake[J].FEMS Microbiology Ecology，1998，25：159-169.
[18]邢德峰，任南琪，宋佳秀，等.不同16S rDNA靶序列对DGGE分析活性污泥群落的影响[J].环境科学，2006，27（7）：1424-1428.（Xing D F，Ren N Q，Song J X，et al.Community of activated sludge based on different targeted sequence of 16S rDNA by denaturing gradient gel electrophoresis[J].Enviroment Science，2006，27(7)：1424-1428.)
[19]温广月，许艳丽，李春杰，等.6株生防细菌对大豆根腐病防治效果初步评价[J].大豆科学，2005，24（2）：121-125.(Wen G Y，Xu Y L，Li C J，et al.Evaluation of six potential biocontrol agents against soybean root rot[J].Soybean Science，2005，24(2)：121-125.)
[20]刘海龙，李春杰，许艳丽.生防细菌的抑菌谱和对大豆根腐病的防治[J].大豆通报，2008，1：10-13.（Liu H L，Li C J，Xu Y L.Antagonistic spectrum of biocontrol bacteria and inhibition to soybean root rot[J].Soybean Bulletin，2008，1：10-13.)
[21] Hu Y S，Wu K，Liu N，et al .Dynamics of microbial communities in bulk and developing cucumber rhizosphere soil[J].Agricultural Sciences in China， 2004，3（5)：376-383.
[22]张炳欣，张平.植物根围外来微生物定殖的检测方法[J].浙江大学学报(农业与生命科学版)，2000，26(6)：624-628.(Zhang B X，Zhang P.Detection of introduced microorganisms to rhizosphere[J].Journal of Zhejiang University（Agric.&Life Sci.)，2000，26(6)：624-628.)
[23]黄海婵，裘娟萍.枯草芽孢杆菌防治植物病害的研究进展[J].浙江农业科学，2005，3：213-215.（Huang H C，Qiu J P.Advance on Bacillus subtilis controlling plant disease[J].Zhejiang Agricultural Science，2005，3：213-215.)

Memo

Memo:
-
Last Update: 2014-09-18