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Molecular Cloning and Expression Analysis of a Polygalac turonase pspg1 fromPhytophthora sojae(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2009年05期
Page:
781-783,790
Research Field:
Publishing date:

Info

Title:
Molecular Cloning and Expression Analysis of a Polygalac turonase pspg1 fromPhytophthora sojae
Author(s):
SUN Wen-xiu
College of Life Science，Yangtze University，Jingzhou 434025，Hubei，China
Keywords:
Phytophthora sojae; Polygalacturonase; Cloning; Expression
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.05.0781
Abstract:
Soybean blight is a worldwide disease，which impacted on agriculture of China and other countries badly，and caused enormous loss on economy.In order to explore the pathogenic role of polygalacturonase from Phytophthora sojae，a polygalacturonase gene pspg1 was cloned from P.sojae by using PCR.And the phylogenetic tree of pspg1 was constructed with PAUP software.Then the expression of pspg1 in soybean was analyzed by RT-PCR.The results showed that the length of open reading frame of pspg1 was 1236 bp，which encoded a protein of 412 amino acid residues.The result of evolutionary analysis indicated that pspg1 was gathered with other pg genes from other oomycetes，which formed an independent branch.More importantly，the evolutionary analysis further defined the status of oomycete in the evolution of the nature. RT-PCR was performed and the results demonstrated that pspg1 expressed in hypocotyl of inoculated soybeans，and the expression was increasing with extending of inoculated time.However，pspg1 was not detected in hypocotyl of healthy soybeans.In this paper，a polygalacturonase gene pspg1 was cloned，and sequence of pspg1 was analyzed.In addition，pspg1 was detected in hypocotyl of inoculated soybeans，which testified that pspg1 played an important role in pathogenic process of P.sojae.

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Last Update: 2014-09-13