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Comparative Analysis of 11S and 7S Protein Relative Content between Wild and Cultivated Soybeans in China(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2009年05期
Page:
759-757
Research Field:
Publishing date:

Info

Title:
Comparative Analysis of 11S and 7S Protein Relative Content between Wild and Cultivated Soybeans in China
Author(s):
LIUShun-hu1; ZHOU Rui-bao2; GAIJun-yi1
1.Soybean Research Institute of Nanjing Agricultural University,National Center for Soybean Improvement,National Key Laboratory for Crop Genetics and Germplasm Enhancement，Nanjing 210095，Jiangsu；
2.Soybean Processing Research Institute，Henan University of Technology，Zhengzhou 450012，Henan；
3.Jining College，Qufu 273155，Shandong，China
Keywords:
Wild soybean（G.soja Sieb.et Zucc.); Cultivated soybean（G.max(L.）Merr.); 11S protein; 7S protein; 11S/7S ratio; Protein subunit group; Genetic variability; Eco-region
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.05.0759
Abstract:
In breeding for cultivars with specific protein quality，it should be the basic step to hold the genetic variability of relative content of 11S，7S protein and their subunit groups in soybean germplasm.In the present study，total 778 accessions，including 138 wild entries（Glycine soja Sieb.et Zucc.)，409 cultivated landraces（Glycine max（L.）Merr.)and 148 released domestic cultivars sampled from various eco-regions as a representative in China，along with 83 released foreign cultivars,were tested for their 11S，7S protein and their subunit group relative contents by using SDS-PAGE analysis.The obtained data were analyzed with the software of SAS 9.0 and SPSS 13.0.The results obtaind under environment conditions in Nanjing are as follows:The mean relative content of 11S in wild accessions，landraces and released cultivars was 54.7%，64.8% and 71.7% with a range of 28.8%-82.6%，38.8%-79.4% and 48.8%-88.9%，respectively.That of 7S was 44.7%，34.9% and 27.9% with a range of 20.6%-71.2%，20.6%-61.1% and 15.7%-47.8%，respectively.That of 11S/7S ratio was 1.4，2.0 and 2.7 with a range of 0.4-3.9，0.6-3.9 and 0.9-4.0，respectively.Due to domestication and artificial breeding，the 11S relative content and 11S/7S ratio increased and the 7S relative content decreased gradually with their ranges all decreased，while the relative contents of subunit group 11S-2 and 11S-3 increased and those of the six 7S subunit groups，especially 7S-1 and 7S-6 decreased.There existed abundant variation in 11S and 7S relative content，11S/7S ratio and their subunit groups in the three germplasm types in each eco-region，but no significant correlation with geographic latitude of accession’s source site，as well as with protein and fat content was found.Eight elite accessions were screened out from the 778 accessions to be with 11S/7S ratio more than 3.7 and 11S relative content 78.9%-88.9%.In addition，there found also the accessions with 11S subunit group relative content more than 24% and the accessions with 11S-1 deficient or one or two 7S subunit groups deficient.These materials should be the superior sources for breeding programs in protein quality improvement.

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Last Update: 2014-09-13