|Table of Contents|

Determination of Genistein in Soybean Isoflavone Sustained-release Pellet Capsules by RP-HPLC(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2009年04期
Page:
727-730
Research Field:
Publishing date:

Info

Title:
Determination of Genistein in Soybean Isoflavone Sustained-release Pellet Capsules by RP-HPLC
Author(s):
REN Yan-rong 1; 2

1College of Bioengineering，Chongqing University，Chongqing 400044；

2Department of Life Science and Chemistry，Chongqing Education College，Chongqing 400067，China

Keywords:
Soybean isoflavone sustained-release pellet capsules; Genistein; RP-HPLC
PACS:
R286.2
DOI:
10.11861/j.issn.1000-9841.2009.04.0727
Abstract:
In order to validly control the quality of soybean isoflavone sustained-release pellet capsules，a method for determination of genistein in it was established using the method of RP-HPLC for determination.Chromatographic conditions include：column:ZOBAX SB-C18（5 μm，250 mm×4.6 mm）；mobile phase：acetonitrile -0.1% H3PO4 solution (40∶60)；detection:UV 262 nm；temperature:35℃;flow rate:1.0 mL min-1；sample size:5 μL.Using one point external standard method to quantitate.Genistein had a good linear relationship in the range of 20.88-208.2 ng，regression equation was Y=4.38X-2.69，R=0.999 (n=6).The average recovery of genistein was 99.97％，RSD=0.87％(n=9).The method is rapid，accurate，reliable and can be used to determine genistein in the soybean isoflavone sustained-release pellet capsules.

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Last Update: 2014-09-24