|Table of Contents|

Development of a Loop-mediated Isothermal Amplification Assay for Rapid Detection of Transgenic Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2009年04期
Page:
706-710
Research Field:
Publishing date:

Info

Title:
Development of a Loop-mediated Isothermal Amplification Assay for Rapid Detection of Transgenic Soybean
Author(s):
LIU Yi1; ZHANG Jun-fang; ZHANG Hui-yan; SU Xu-dong; MA Xiao-yan; KANG Chun-yu; WANG Xue-jing; ZHANG Wei
Institute of Food Science and Technology，Agricultural University of Hebei，Baoding 071001，Hebei，China
Keywords:
Loop-mediated isothermal amplification; Detection; CP4-EPSPS; Transgenic soybean
PACS:
s565.1
DOI:
10.11861/j.issn.1000-9841.2009.04.0706
Abstract:
A loop-mediated isothermal amplification(LAMP) technology was established to detect transgenic soybean.The sequence of exogenous gene CP4-EPSPSwas used as target sequence，to design outer primers，inner primers and loop primers.The DNA extraction method was improved.Through visual observation of white precipitate to judge results of detection.The improved CTAB method isolated total DNA quickly and reducing DNA0extraction cost as compared with the traditional CTAB method.The detection limit of the genetically modified soybeans by LAMP technology was 0.01% which was superior to PCR.Results indicate that LAMP can provide a sensitive，rapid yet simple test for the detection of CP40EPSPS in transgenic soybean.

References:

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Last Update: 2014-09-24