|Table of Contents|

Effect of Different Forms Nitrogen on Nodule Growth and Nitrogen Fixation in Soybean (Glycine max L.)(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2009年04期
Page:
674-677
Research Field:
Publishing date:

Info

Title:
Effect of Different Forms Nitrogen on Nodule Growth and Nitrogen Fixation in Soybean (Glycine max L.)
Author(s):
YAN Jun1; 2; HAN Xiao-zeng1; 2; WANG Shou-yu1; WANG Shu-qi1; LI Xiao-hui1
1.Northeast Institute of Geography and Agricultural Ecology，Chinese Academy of Sciences,Harbin 150081，Heilongjiang；
2 .College of Agriculture，Northeast Agricultural University，Harbin 150030，Heilongjiang，China
Keywords:
Nodule; Nitrogenase activity; Different forms nitrogen; Soybean
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.04.0674
Abstract:
Nitrogen plays an important role in nitrogen fixation of soybean.In this paper，a pot culture experiment was carried out to study the effects of different forms nitrogen on the variation of dry weight (DW)，number，nitrogen content，nitrogen accumulation and nitrogenase activity in nodule of soybean (Glycine max L.) at flowering stage.The results showed that different forms nitrogen promoted nodule formation and growth，which embodied in dry weight and number significantly increased compared with biological nitrogen fixation.The order of the nodule DW and nodule number were NO+-4-N(N2)>Protein-N(N4)>NO-3-N(N1)>Amino Acid-N(N3)>Amide-N(N5)>Biological Nitrogen Fixation(N0)，the least nodule DW and number appeared in N0 treatment，while the most occurred in N2 treatment.Meanwhile different forms nitrogen increased N content and N accumulation in nodule.The trend of the nitrogen content in nodule was as follows：N1>N4>N5>N2>N3>N0，and nitrogen content was the highest in the N1 treatment，moreover，the difference among N0，N1，N4，N5 was significan at 5% level.However，the order of the N accumulation was N2>N4>N1>N5>N3>N0.The different forms nitrogen remarkably affected nitrogenase activity，the trend was N0>N5>N3>N2>N4>N1，and the nitrogenase activity was the highest in N0 treatment.Therefore，different forms nitrogen had different effects on nitrogen fixation of soybean.

References:

[1] O’hwaki Y，Sugahara P.Active extrusion of protons and exudation of carboxylic acids in response to iron deficiency by roots of chickpea (Cicer arietinum L.)[J].Plant Soil，1997，189：49-55.

?[2]桑原真人.タィズの多收条件と窒素代谢(2)[J].农业ぉょぴ园艺，1986，61(5):590-598.(Setiyono.Environment conditions and nitrogen metabolization of soybean[J].Journal of Agronomy and Horticulture，1986，61(5)：590-598.)
[3]藤田耕之辅.タィズにょけゐ窒素の固定、吸收、转流に对すゐ化合态窒素の影响[J].土壤肥料学杂志，1982，53(1):30-34.(Takahasuki.Chemical nitrogen on nitrogen fixation，absorption and transfer of soybean[J].Journal of Soil and Fertilizer，1982，53(1)：30-34.)
[4]但野利秋，田中明.アソモニア态窒素および硝酸态窒素适应性の作物种间差[J].日本土壤肥料学杂志，1976，47 (7) ：321-328.(Tadano T，Tanaka M.Difference of adaptability to ammonium and nitrate in different crop varieties[J].Japanese Journal of Soil and Fertilizer Sciences，1976，47 (7):321-328.)
[5]宋海星，申斯乐，马淑英，等.硝态氮和铵态氮对大豆根瘤固氮的影响[J].大豆科学，1997，16(4):283-286.(Song H X，Shen S L，Ma S Y，et al.Effect of NO- 3-N and NH+-4-N on the nitrogen fixation of soybean nodules[J].Soybean Science,1997，16(4)：283-286.)
[6]吴良欢，陶勤南.水稻氨基酸态氮营养效应及其机理研究[J].土壤学报，2000，37(4):464-473.(Wu L H，Tao Q N.Effects of amino acid-N on rice nitrogen nutrition and its mechanism[J].Acta Pedologica Sinica.,2000，37(4)：464-473.)?
[7]莫良玉.高等植物氨基酸态氮营养效应研究[D].杭州：浙江大学，2001，24-66.(Mo L Y.Studies on the effects of amino acid nitrogen on different plants under sterile culture [D].Hangzhou：Zhejiang University，2001，24-66.)
[8]张政.氨基酸态氮对黄瓜的营养效应[D].重庆：西南农业大学，2005，28-30.(Zhang Z.Effects of amino acid nitrogen on cucumber growth [D].Chongqing：Southwest Agricultural University，2005，28-30.)
[9] M.奥巴托.共生固氮技术手册：豆科植物-根瘤菌[M].北京：科学出版社，1987.(M.Aobato.The manual of symbiotic nitrogen fixation：legumes plant-rhizobium[M].Beijing：Science Press，1987.)
[10]鲁如坤.土壤农业化学分析方法[M].北京：中国农业科技出版社，2000.(Lu R K.Soil agricultural chemical analysis methods [M].Beijing：Chinese Agricultural Science and Technology Press，2000.)
[11]周朝晖，颜文斌，张凤章，等.固氮酶催化作用机理及其化学模拟[J].Journal of Xiamen University(Natural Science)，2001，40(2):320-329.(Zhou Z H，Yan W B，Zhang F Z.Catalytic mechanism of nitrogen reduction by nitrogenase and its chemical modeling[J].Soybean Science，2001，40(2)：320-329.)
[12]苗淑杰，乔云发，韩晓增.两种形态氮源条件下磷对大豆结瘤固氮的影响[J].大豆科学，2006，25(3):250-278.(Miao S J，Qiao Y F，Han X Z.Effects of P deficiency on nodulation and function in soybean under two different N sources[J].Soybean Science,2006，25(3)：250-278.)
[13]孙彦浩，陈殿绪，张礼凤.花生施氮肥效果与根瘤菌固N的关系[J].中国油料作物学报，1998，20(3):69-72.(Sun Y H，Chen D X，Zhang L F.The relationship between the effect of N application and nitrogen fixation in peanut[J].Chinese Journal of Oil Crop Sciences,1998，20(3)：69-72.)
[14] Marschner H.Mineral nutrition of higher plant[J].Academic press,Inc.，London，UK,1986:674.
[15]甘银波，陈静，邱正明，等.不同阶段施用氮肥对大豆氮吸收及固氮的影响[J].中国油料，1996，18(4):45-48.(Gan Y B，Chen J，Qiu Z M，et al.Effect of N fertilizer application at different growth stages on uptake and N fixation of soybean[J].Journal of Chinese Oils，1996，18(4)：45-48.)

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Last Update: 2014-09-22