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Relationship between the Contents of Soybean Isoflavanones and Relative Expression Quantity of PAL Gene(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2009年04期
Page:
670-673
Research Field:
Publishing date:

Info

Title:
Relationship between the Contents of Soybean Isoflavanones and Relative Expression Quantity of PAL Gene
Author(s):
ZHANG Da-yong; LI Wen-bin; LI Dong-mei; LI Yong-guang
Soybean Institute，Northeast Agricultural University，Harbin 150030，Heilongjiang，China
Keywords:
Isoflavones; PAL; Relative expression quantity
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.04.0670
Abstract:
The phenylalanine ammonialyase(PAL)is an access point enzyme and also one of isoflavone synthesis key enzyme in the phenylalanine pathways.In order to study the relationship between the contents of soybean isoflavanones and relative expression quantity of PAL gene，a sand culture experiment was conducted.Soybean leaves at different growth stage were sampled，and the isoflavone content were measured and the relative expression quantity of alanin ammonialyase(PAL)gene were determined by RQ-PCR.There was a coordination fluctuation tendency between phenylalanin ammonialyase(PAL)gene relative expression quantity and isoflavone content in soybean leaf.Soybean isoflavones content and relative expression quantity of phenylalanin ammonialyase(PAL)gene appeared a peak value at R1 stage.

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Last Update: 2014-09-22