|Table of Contents|

A RT-PCR Method Suitable for MicroRNA Identification of Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2009年04期
Page:
600-604
Research Field:
Publishing date:

Info

Title:
A RT-PCR Method Suitable for MicroRNA Identification of Soybean
Author(s):
LIU Yong-xin1; HAN Ying-peng1; CHANG Wei1; ZOU Quan2; GUO Mao-zu2; LI Wen-bin1
1Soybean Institute，Northeast Agricultural University；Chinese Education Ministry’s Key Laboratory of Soybean Biology，Harbin 150030，Heilongjiang
2.Department of Computer Science and Technology，Harbin Institute of Technology，Harbin 150001，Heilongjiang，China
Keywords:
Soybean; MicroRNA; RT-PCR; Identification
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.04.0600
Abstract:
The microRNAs (miRNAs) are a newly identified class of small non-coding RNA，which regulate post-transcriptional gene expressing in eukaryote.Most miRNAs are predicted through the computer，the presentence/absence and the spatial/temporal of miRNAs still unclear.It is important for the establishment of efficient，sensitive and specific detection methods to detect miRNAs in different organism or period.In this study，the protocol derived from Lau et al.and Shi et al.was used to detect miR156a，predicted and released by Sanger Institute，in old or young leaf，root，and shoot for verifying this method.By comparison，this method is low cost，simple，fast，and sensitive，common for primer of RT-PCR，which could identify a number of miRNAs in the same organism and compare miRNAs from different organism.Furthermore，this method need less RNA，in comparison with Northern Blot and Microarray.

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Last Update: 2014-09-21