|Table of Contents|

An Integrated Genetic Linkage Map of Soybean and Its Application(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2009年04期
Page:
557-565,573
Research Field:
Publishing date:

Info

Title:
An Integrated Genetic Linkage Map of Soybean and Its Application
Author(s):
ZHOU Bin1XING Han1CHEN Shou-yi2GAI Jun-yi1
1Soybean Research Institute of Nanjing Agricultural University,National Center for Soybean Improvement,State Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing 210095,Jiangsu;
2The State Key Laboratory of Plant Gemomics,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Being 100101,China
Keywords:
Key words:Soybean genetic linkage mapIntegrationSSR
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.04.0557
Abstract:
Integration of genetic linkage maps into a joint one is an effective approach to overcome the limitation of number of markers due to the constraint of polymorphism in individual specific mapping populations.In the present study,four RIL populations,Kefeng 1×Nannong 1138-2,Nannong 87-23×NG94-156,Su 88-M21×Xinyixiaoheidou and Wan 82-178×Tongshanbopihuangdoujia derived from the crosses between distinct elite cultivars of Glycine max(L.)Merr.were used at first to construct individual genetic linkage maps with 560,223,195 and 133 markers,respectively,by using the software JoinMap 3.0.Then based on the common SSR markers across the four maps,the individual maps were integrated into an integrated genetic linkage map by using the same software,which containing 795 markers spanning 2 772.9 cM of the soybean genome,distributed on 20 linkage groups with the length of linkage groups varied from 77.1 cM to 224.7 cM,the marker number from 24 to 69,and an average marker distance of 3.49 cM.Among the linkage groups,C2,C1,N and F are obviously highly enhanced.In comparison with Song et al’s integrated map,the present map shows a good coincidence with it except with six SSR markers located on different linkage groups and five new SSR markers added to the present map.The integrated map can be used in locating genes/QTLs detected in marker analysis and association mapping,in comparing mapping results from different populations,and in searching for closer markers.The present integrated map performed a reasonable result in comparison with the individual maps.Therefore,the present map is potential in QTL mapping study,especially for domestic soybean breeding purposes since the parental materials of the four RIL populations are closely related to the breeding materials in Chinese breeding programs.

References:

[1]Cregan P B,Jarvik T,Bush A L,et al.An integrated genetic linkage map of the soybean genome[J].Crop Science,1999,39(5):1464-1490.

[2] Song Q J,Marek L F,Shoemaker R C,et al.A new integrated genetic linkage map of the soybean[J].Theoretical and Applied Genetics,2004,109(1):122-128.
[3] Xia Z,Tsubokura Y,Hoshi M,et al.An integrated high-density linkage map of soybean with RFLP,SSR,STS,and AFLP markers using a single F2?population[J].DNA Research,2007,14:257-269.
[4] Yamanaka N,Nagamura Y,Tsubokura Y.Quantitative trait locus analysis of owering time in soybean using a RFLP linkage map[J].Breeding Science,2000,50:109-115.
[5] Yamanaka N,Ninomiya S,Hoshi M.An informative linkage map of soybean reveals QTLs for flowering time,leaflet morphology and regions of segregation distortion[J].DNA Research,2001,8:61-72.
[6]庄炳昌,陈受宜.大豆遗传图谱的构建和分析[J].遗传学报,2000,27(11):1018-1026.(Zhuang B C,Chen S Y.Construction and analysis of soybean genetic map[J].Acta Genetic Sinica,2000,27(11):1018-1026.)
[7]吴晓雷,贺超英,王永军,等.大豆遗传图谱的构建和分析[J].遗传学报,2001,28(11):1051-1061.(Wu X L,He C Y,Wang Y J,et al.Construction and analysis of a genetic linkage map of soybean[J].Acta Genetic Sinica,2001,28(11):1051-1061.)
[8]王永军,吴晓雷,贺超英,等.大豆作图群体检验与调整后构建的遗传图谱[J].中国农业科学,2003,36(11):1254-1260.(Wang Y J,Wu X L,He C Y,et al.A soybean genetic linkage map constructed after the mapping population being tested and adjusted[J].Scientia Agricultura Sinica,2003,36(11):1254-1260.)
[9] Zhang W K,Wang Y J,Luo G Z,et al.QTL mapping of ten agronomic traits on the soybean (Glycine max(L.) Merr.) genetic map and their association with EST markers[J].Teoretical and Applied Genetics,2004,108(6):1131-1139.
[10] Fu S X,Zhan Y,Zhi H J,et al.Mapping of SMV resistance gene Rsc-7 by SSR markers in soybean[J].Genetica,2006,128(1):63-69.
[11]宛煜嵩,王珍,肖英华,等.一张含有227个SSR标记的大豆遗传连锁图[J].分子植物育种,2005,3(1):15-20.(Wan Y S,Wang Z,Xiao Y H,et al.A soybean genetic linkage map comprising of 227 SSR loci in a soybean RIL population [J].Molecular Plant Breeding,2005,3(1):15-20.)
[12]巩鹏涛,木金贵,赵金荣,等.一张含有315个SSR和40个AFLP标记的大豆分子遗传图的整合[J].分子植物育种,2006,4(3):309-316.(Gong P T,Mu J G,Zhao J R,et al.An integrated soybean genetic linkage map comprising 315 SSRs and 40 RFlPs [J].Molecular Plant Breeding,2006,4(3):309-316.)
[13] Morgante M,Olivieri A M.PCR-amplified microsatellites as markers in plant genetics[J].The Plant Journal,1993,3(1):175-182.
[14] Morgante M,Rafalski A,Biddle P,et al.Genetic mapping and variability of seven soybean simple sequence repeat loci[J].Genome,1994,37(5):763-769.
[15] Bell C J,Ecker J R.Assignment of 30 microsatellite loci to the linkage map of Arabidopsis[J].Genomics,1994,19:137-144.
[16] Senior M L,Murphy J P,Goodman M M,et al.Utility of SSRs for determining genetic similarities and relationships in maize using an agarose gel system[J].Crop Science,1998,38:1088-1098.
[17] Burnham K D,Francis D M,Dorrance A E,et al.Genetic diversity patterns among phytophthora resistant soybean plant introductions based on SSR markers[J].Crop Science,2002,42:338-343.
[18] Poland J A.Mapping of Aegilops tauschii derived leaf rust resistance genes in common wheat[J].Journal of Natural Resources and Life Science Education,2003,32:8-11.
[19] Lander E S,Green P,Abrahamson J,et al.Mapmaker:An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations[J].Genomics,1987,1(2):174-181.?
[20] Lincoln S E,Lander S L.Mapmaker/exp 3.0b and Mapmaker/QTL 1.1[CP].Whitehead Institute of Medical Research,Cambridge,Massachusetts,1993.
[21] Ooijen J W V,Voorrips R E.Joinmap:Software for the calculation of genetic linkage maps[CP].Plant Research International,Wageningen,the Netherlands,2001.
[22]吕祝章.大豆遗传图谱构建、重要农艺性状QTL定位及优异基因发掘[D].泰安:山东农业大学,2006.(Lü Z Z.Construction of soybean genetic map,QTL mapping of agronomic traits,and identification on excellent gene[D].Taian:Shandong Agricultural University,2006.)
[23] Mansur L M,Orf J H,Chase K.Genetic mapping of agronomic traits using recombinant inbred lines of soybean[J].Crop Science,1996,36:1327-1336.
[24]王春娥,盖钧镒,傅三雄,等.大豆豆腐和豆乳得率的遗传分析与QTL定位[J].中国农业科学,2008,41(05):1274-1282.(Wang C E,Gai J Y,Fu S X,et al.Inheritance and QTL mapping of Tofu and soymilk output in soybean[J].Scientia Agricultura Sinica.2008,41(5):1274-1282.)
[25]张红梅,周斌,赵团结,等.大豆重组自交系群体NJRISX豆腐和豆乳得率的QTL分析[J].作物学报,2008,34(1):67-75.(Zhang H M,Zhou B,Zhao T J,et al.QTL mapping of Tofu and soymilk output in RIL population NJRISX of soybean[J].Acta Agronomica Sinica,2008,34(1):67-75.)
[26]文自翔,赵团结,郑永战,等.中国栽培和野生大豆农艺品质性状与SSR标记的关联分析[J].作物学报,2008,34(7):1169-1178.(Wen Z X,Zhao T J,Zheng Y Z,et al.Association analysis of agronomic and quality traits with SSR markers in Glycine max and Glycine soja in China:Ⅰ.population structure and associated markers[J].Acta Agromomica Sinica,2008,34(7):1169-1178.)
[27]张军,赵团结,盖钧镒.大豆育成品种农艺性状QTL与SSR标记的关联分析[J].作物学报,2008,34(12):2059-2069.(Zhang J,Zhao T J,Gai J Y.Association analysis of agronomic trait QTLs with SSR markers in released soybean cultivars[J].Acta Agromomica Sinica,2008,34(12):2059-2069.)

Memo

Memo:
-
Last Update: 2014-09-21