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A Comparative Study on Soybean Leaf Proteomics under Different Photoperiod Treatments(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2009年03期
Page:
388-393
Research Field:
Publishing date:

Info

Title:
A Comparative Study on Soybean Leaf Proteomics under Different Photoperiod Treatments
Author(s):
HE WeiJIANG Zhen-fengZHAO LinHAN Ying-pengLI Wen-bin
Soybean Research Institute of Northeast Agricultural University,Key Laboratory of Soybean Biology of Chinese Education Ministry,Harbin 150030,Heilongjiang,China
Keywords:
SoybeanTwo-dimensional gel electrophoresis(2-DE)ProteomicsPhotoperiodLiquid and ion trap mass spectrometry
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.03.0388
Abstract:
Dongnong 42,a photoperiod sensitive cultivar,was treated with 8 h and 16 h light illuminations,respectively.The leaf tissues were then sampled and two-dimensional gel electrophoresis(2-DE)was performed to separate the protein spots.The gels were stained with Coomassie Blue G-250.The difference between the protein maps of leaves from two different photoperiod treatments was analyzed with the PDQuest image software(Bio-rad).The liquid and ion trap mass spectrometry(LC-ESI-MS)was used to obtain the MS/MS of the differentially expressed proteins and the MASCOT software was used to search the protein database.A total of 16 differentially expressed proteins were identified,of them nine proteins showed up-regulation expression and four showed down-regulation expression.The major differentially expressed proteins,glutathione-S-transferase,disulfide isomerase,metalloproteinases,TCTP,Rieske iron-sulphur protein precursor cysteine synthetic enzymes,were reviewed and discussed.These proteins might be related to plant photosynthesis,signal transduction and apoptosis control,detoxification and plant defense and other physiological and biochemical function.

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Last Update: 2014-09-25