|Table of Contents|

Molecular Mechanism of A5A4B3null and A3B4null of Chinese Soybean Germplasm(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2009年03期
Page:
363-369
Research Field:
Publishing date:

Info

Title:
Molecular Mechanism of A5A4B3null and A3B4null of Chinese Soybean Germplasm
Author(s):
LIU Zhong-yue1; WANG Hong-ling1; 2; LIU Chun1; 3; MA Hao1
1State Key Laboratory of Crop Genetics and Germplasm Enhancement，Soybean Research Institue，Nanjing Agricultural University，Nanjing 210095,Jiangsu；
2Institute of Genetics and Breeding，Forest Academy of Jiangsu Province，Nanjing 211153,Jiangsu；
3Department of Life Science，Hengyang Normal University，Hengyang 421008,Hunan,China
Keywords:
Soybean; A5A4B3subunit; A3B4?subunit; Subunit lacking; Point mutation of codon
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.03.0363
Abstract:
There is great genetic diversity in the relative content of seed storage protein subunits in Chinese soybean germplasm，which is the foundation of soybean protein quality improvement.Therefore，it will be helpful for soybean high-quality protein breeding to understand the molecular mechanism of the subunit mutations.Based on the validation of their subunit deficiency by sodium dodecyl sulfate-polyacrylamide gel electrophoresis（SDS-PAGE)，seven landraces without A5A4B3and two without A3B4subunit were used as experimental materials and their subunit mutant gene sequences and cDNA sequences were obtained.Compared with normal sequences on NCBI，the start codon ATG of the genes and cDNAs encoding the A5A4B3subunits of seven mutant landraces was found to mutate to ATA，which produced a fire-new reading frame of translation and resulted in subunit lacking.While the stop codon TAA of cDNAs encoding A3B4subunit of two mutant landraces were found to mutate to CAA，which may resulted in an additional tail in pro-glycinin and caused subunit lacking.

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Last Update: 2014-09-25