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Enhanced Ultraviolet-B Radiation Reduced Isoflavones Contents in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2009年02期
Page:
357-359
Research Field:
Publishing date:

Info

Title:
Enhanced Ultraviolet-B Radiation Reduced Isoflavones Contents in Soybean
Author(s):
LI Xian-jia; SONG Qing-huan; REN Li-ping
Luohe Medical College,Luohe 462002， Henan，China
Keywords:
UV-B radiation; Soybean isoflavones; HPLC
PACS:
R284
DOI:
10.11861/j.issn.1000-9841.2009.02.0357
Abstract:
The effect of enhanced ultraviolet radiation(UV-B) on soybean isoflavone content was investigated, a high performance liquid chromatography(HPLC) method for the determination of soybean isoflavones was presented. Soybean plants were treated with enhanced UV-B till mature．The harvested soybean seeds were extracted by ultrasonic assisting and then separated on BDS Hypersil C18 column and methanol-0.4％ phosphoric acid solution (30∶70,by volum)．The elute was detected by a UV detector at 254 nm. Results showed the 180 mwm-2?UV-B treatment significantly affects soybean isoflavones，Daidzin，Glycitin，Genistin and Daidzeint decreased by 4.5%，8.6％，1.1% and 11.4％,respectively．The increase of UV-B treatment and the decrease of isoflavones content had good linearity.

References:

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Last Update: 2014-10-04