|Table of Contents|

A Two-Dimensional Electrophoresis Protocol Suitable for Proteomic Analysis of Soybean Leaves(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2009年02期
Page:
325-328
Research Field:
Publishing date:

Info

Title:
A Two-Dimensional Electrophoresis Protocol Suitable for Proteomic Analysis of Soybean Leaves
Author(s):
WANG Xue; DUAN Yu-xi; CHEN Li-jie
Shenyang Agricultural University，Nematology Institute of Northern China，Shenyang 110161,Liaoning,China
Keywords:
Soybean; Leaf; proteome; Two-dimensional electrophoresiss
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.02.0325
Abstract:
Proteomics is becoming a necessity in plant biology，as it is in medicine，zoology and microbiology.This study aimed at solving the problem of low content of protein in soybean leaf and the disturbance of secondary metabolites such as chromatophore，phenols and quinones to the separation of two-dimensional electrophoresis.Using leaves of soybean as material，index such as protein extraction，lysis buffer and IEF parameter were modified and improved .Results showed that，soybean leaves protein was extracted with TCA/acetone，then resolubilized in lysis buffer（9 molL-1urea，2 molL-1thiourea，100 mmolL-1DTT，0.5% Bio-Lyte），Isoelectric focusing was performed at 17℃ under the 24000Vh conditions.A 2-DE（two-dimensional electrophoresis）protocol suited for the separation of proteins from soybean leaves was elementary established.

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Last Update: 2014-10-04