|Table of Contents|

Effect of Soybean Isoflavone Aglycones on Proliferation and Apoptosis of Colon Carcinoma Cell HT(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2009年02期
Page:
310-313
Research Field:
Publishing date:

Info

Title:
Effect of Soybean Isoflavone Aglycones on Proliferation and Apoptosis of Colon Carcinoma Cell HT
Author(s):
WANG Xia; XU Hui-xian; QUAN Ji-shu; YIN Xue-zhe
Yanbian University Health Science Center，Yanji 133000，Jilin，China
Keywords:
Soybean; Isoflavones; Aglycone; Colon cancer; Apoptosis
PACS:
R282.7
DOI:
10.11861/j.issn.1000-9841.2009.02.0310
Abstract:
Soybean isoflavones have been observed to limit the proliferation of tumor cell by inducing apoptosis.Soybean isoflavone glycosides were extracted from soybean hypocotyl，then isoflavone aglycones were prepared by hydrolysis of isoflavone glycosides and its inhibitory effect on proliferation of colon carcinoma cell was investigated.MTT assay was used to examine the proliferation of HT-29 cell，TUNEL method was used for analysis of cell apoptosis，and immunohistochemical method was used for the determination of bax，bcl-2 and p53 expression.The results showed that soybean isoflavone aglycones inhibited the proliferation and induced apoptosis of colon carcinoma HT-29 cell in a time- and concentration-dependent manner in the concentration of 20-80 mgL-1.Inhibitory ratio of isoflavone aglycones on carcinoma cell proliferation was(57.1±4.9)% and apoptosis ratio was(20.9±2.1)%.Immunohistochemical results also showed that isoflavone aglycones significantly increased bax protein expression and decreased bcl-2 level.It is suggested that soybean isoflavone aglycones could exert an anti-colon cancer effect via induction of apoptosis.

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Last Update: 2014-10-04