|Table of Contents|

Detection of Genetically Modified Soybean and Products by LAMP Reaction(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2009年02期
Page:
305-309
Research Field:
Publishing date:

Info

Title:
Detection of Genetically Modified Soybean and Products by LAMP Reaction
Author(s):
LIU Cai-xiaLIANG Cheng-zhuXU BiaoGAO Hong-weiLIN ChaoSUN Min
Technical Center of Inspection and Quarantine,Shandong Administration for Entry-Exit Inspection and Quarantine,Qingdao 266002,Shandong China
Keywords:
Loop mediated isothermal amplificationRapid detectionRoundup Ready EPSPS gene
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.02.0305
Abstract:
The loop-mediated isothermal amplification(LAMP)that amplifies DNA with high specificity and rapidity under an isothermal condition was applied for rapid detection of Roundup Ready and its products.A set of four primers,two outer and two inner primers,was designed specifically to recognize EPSPS gene of Roundup Ready.The LAMP reaction mix was optimized.The optimal reaction temperature and time of the LAMP assay for EPSPS gene were 65℃ and 1 h,respectively.The LAMP detection method has been secessfully set up.The results showed that the LAMP reaction system can detect EPSPS gene effectively and sensitively.The limit of detection is under 0.01% lower than the international limit.These results suggest that detection of EPSPS by LAMP is an effective and low-cost procedure with high specificity and sensitivty that requires no specialized equipment.This asssy is expected to become a valuable tool for rapid detection and identification of Roundup Ready soybean and its products.

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Last Update: 2014-10-04