|Table of Contents|

Purification of β Subunit from β-conglycinin and Identification of Immunological Activity of the Prepared Subunit(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2009年02期
Page:
301-304,309
Research Field:
Publishing date:

Info

Title:
Purification of β Subunit from β-conglycinin and Identification of Immunological Activity of the Prepared Subunit
Author(s):
ZHENG Shu-gui1; 2; CAO Song-yi3; SUN Ze-wei1; QIN Gui-xin1
1College of Animal Science and Technology，Jilin Agricultural University，Changchun 130118，Jilin;
2College of Animal Husbandry and Veterinary Medicine，Shenyang Agricultural University，Shenyang 110161，Liaoning;
3College of Biological Technology，Shenyang Agricultural University，Shenyang 110161，Liaoning，China
Keywords:
β-conglycinin; β subunit; Purification; Immunological activity
PACS:
TQ936.21+2
DOI:
10.11861/j.issn.1000-9841.2009.02.0301
Abstract:
Purified β-conglycinin prepared by electric-point precipitation and filtration chromatography was denatured by 6 molL-1urea and separated into free subunits.The subunits of β-conglycinin were isolated and purified by DEAE-Sepharose F F anion exchange chromatography and the prepared subunits were renatured by dialysis.The rabbits were immunized by β-conglycinin to produce β-conglycinin special antiserum.The immune responses between purified subunits and the antiserum were determined by immunoblotting.The results indicated：pure β subunit could be prepared from β-conglycinin by DEAE-Sepharose F F anion exchange chromatography，and the purified β subunit could specially bind to β-conglycinin antiserum and this proved that the subunit had immunological activity.

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Last Update: 2014-10-04