|Table of Contents|

Establishment of an Event-specific Qualitative PCR Method for Detection of Roundup RReady2YieldTM(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2009年02期
Page:
296-300
Research Field:
Publishing date:

Info

Title:
Establishment of an Event-specific Qualitative PCR Method for Detection of Roundup RReady2YieldTM
Author(s):
LI Fei-wu; LI Cong-cong; XING Zhen-juan; LIU Na; KANG Ling-sheng; SONG Xin-yuan; SHAO Gai-ge; ZHANG Ming
Jilin Academy of Agricultural Sciences，National Center for Environmental Safety Inspection of Transgenic Plants，Ministry of Agriculture，Changchun 130033，Jilin，China
Keywords:
Genetically modified organism; Roundup RReady2YieldTM; Event-specific PCR
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.02.0296
Abstract:
Roundup RReady2YieldTM(RR2Y)is a glyphosate-tolerant genetically modified soybean，which is approved to be imported and used as raw material in China.For labeling them according to the regulations on safety of agricultural genetically modified organisms，an event-specific qualitative PCR method for RR2Y is urgently needed to be established，which is the aim of this study.The specific primers were designed based on the 5′-junction sequences of the exogenous integrant of RR2Y，and amplification products of 223 bp were obtained.The ruggedness，specificity，sensitivity，stability and repeatability of this method were tested.The results showed that RR2Y event can be distinguished specifically from other GM and non-GM crops by using this method，and the limit of detection is up to 0.05%.

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Last Update: 2014-10-04