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Effect of Boron on Cell Structure and Shape in Soybean(Glycine max)Callus(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2009年02期
Page:
225-228
Research Field:
Publishing date:

Info

Title:
Effect of Boron on Cell Structure and Shape in Soybean(Glycine max)Callus
Author(s):
GE Zheng-zhen1; YANG Guang-yu2; ZHONG Yan1; DU Qing-zhen1; MENG Xiang-xun1
1School of Basic Medicine and Biological Science，Soochow University，Suzhou 215123，Jiangsu;
2 Soybean Research Center ，Jilin Academy of Agricultural Sciences ，Changchun 130033，Jilin，China
Keywords:
Soybean(Glycine max Merr); Boron; Callus; Cell structure; Cell shape
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.02.0225
Abstract:
Boron is one of the essential microelements for plant growth，playing an important role in maintaining the structural and functional integrity of cell wall and cellular membrane.In this experiment the influences of excess boron on structure and shape of callus cells and the regulatory function of boron in callus induction and regeneration were studied using the successfully induced soybean callus.The medium with various content of boric acid were used to culture soybean tissue of hypocotyledonary axis to induce the callus and successive cell generations.Our results suggested that some callus on the medium with over 70 mgL- 1 boron was loosen and its cells were rarefaction after twenty day-culture.When this callus was transferred into the normal medium MS+2，4-D 0.4 mgL- 1?for a subsequent culture，it was observed that the cells of the callus still had the capability of division and the structure of the callus became looser and more unicells appeared with various cell shapes.

References:

[1]祖艳群，林克惠.硼在植物体中的作用及对作物产量和品质的影响[J].云南农业大学学报，2000，15(4)：359-363.（Zu Y Q，Lin K H.The role of boron in plants and its effect on the yield and quality of crops[J].Journal of Yunnan Agricultural University，2000，15(4)：359-363.）

[2]吴静，李春俭，江荣风，等.缺硼对大豆植株生长和根瘤固氮活性的影响[J].华北农学报，1999，14(2)：1-5.（Wu J，Li C J，Jiang R F，et al.Effects of boron-deficiency on soybean growth and nitrogenase activity[J].Acta Agriculturae Boreali-Sinica，1999，14(2)：1-5.）
[3]Cheng C，Rerkasem B.Effects of boron on pollen viability in wheat[J].Plant and Soil，1993，155-156(1)：313-315.
[4]刘鹏.大豆钼、硼营养研究进展[J].中国农业通报，2001，17(6)：41-44，118.（Liu P.The research development of molybdenum & boron nutrition in soybean[J].Chinese Agricutural Science Bulletin，2001，17(6)：41-44，118.）?
[5]Bolaos L，Lukaszewski K，Bonilla I，et al.Why boron?[J].Plant Physiology and Biochemistry，2004，42(11):907-912.
[6]Maoth T.Boron in plant cell walls[J].Plant and Soil，1997，193(1-2)：59-70.
[7]Kakegawa K，Ishii T，Matsunaga T.Efferts of boron deficiency in cell suspension cultures of Populus alba L.[J].The Plant Cell Reports，2005，23(8)：573-578
[8]刘海坤，卫志明.一种大豆成熟种子的消毒方法[J].植物生理学通讯，2002，38(3)：260-261.(Liu H K，Wei Z M.A method for sterilizing mature seeds of soybean[J].Plant Physiology Communication，2002，38(3)：260-261.)
[9]刘鹏，徐根娣，赵娅儿.锰、硼对大豆几种生理效应的影响[J].中国油料作物学报，2003，25(4)：73-76.（Liu P，Xu G D，Zhao Y E.Effert of boron and manganese on same plysiological characteristics of soybean[J].Chinese Journal of Oil Crop Sciences，2003，25(4)：73-76.

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Last Update: 2014-10-03