|Table of Contents|

Transfer of a GUS gene Cassette into Soybean via Ovary-drip Transformation(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2009年02期
Page:
191-194
Research Field:
Publishing date:

Info

Title:
Transfer of a GUS gene Cassette into Soybean via Ovary-drip Transformation
Author(s):
LIU Jian-feng1; SU Qiao1; AN Li-jia1; RAN Xue-zhong2
1Department of Bioscience and Biotechnology，Dalian University of Technology，Dalian 116024，Liaoning;
2Tieling Academy of Agricultural Sciences,Tieling 112616,Liaoning,China
Keywords:
Soybean; Vector-free; Marker-free; GUS gene cassette; Ovary-drip; Transformation
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.02.0191
Abstract:
To validate the reproducibility and genetic stability of the ovary-drip transformation，the GUS gene cassette，which was composed of only the GUS gene，expression regulatory sequence(35S CaMV promoter，NOS terminator)，and T-DNA border sequence at both sides，was introduced into soybean.The results(Histochemistry GUSanalysis and PCR screening of T0 generation)showed that 12 out of 340 detected immature embryo were GUS positive and six out of 180 transformed plants were PCR positive and GUS positive，the rate of the T0generation transformation reached 3.53% and 3.33%，respectivly.Southern blot analysis confirmed that GUS gene was integrated into the soybean genome with low copy numbers.The GUS staining，PCR analysis and Northern blot indicated that GUS gene was inherited in the transgenic progenies.Moreover，Mendelian segregation was observed in the S2 line.

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Last Update: 2014-10-03