|Table of Contents|

Transformation of -Bt(cryⅠA)-Gene into Soybean via Agrobacterium-mediated Method(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2009年02期
Page:
186-190，194
Research Field:
Publishing date:

Info

Title:
Transformation of -Bt(cryⅠA)-Gene into Soybean via Agrobacterium-mediated Method
Author(s):
QU Shan-shan1; 2; LIU Li-jun1; KOU Kun1; 2; TANG Xiao-fei1; YANG Zhe1
1Soybean Research Institute，Heilongjiang Academy of Agricultural Sciences，Harbin 150086，Heilongjiang；
2Graduate School of Northeast Agricultural University，Harbin 150030，Heilongjiang，China
Keywords:
Soybean; Bt gene; Genetic transformation
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.02.0186
Abstract:
It is very important for diseases and insect pests controlling to transfer some resistant genes to obtain transgenic resistant varieties.In this study，plant expression vector pCAMBIA3300-Bt containing -Bt（cryⅠA）- gene was constructed，and transgenic plants were obtained by Agrobacterium-mediated transformation of soybean(Heinong 37) cotyledonary nodes.We regenerated plants and optimized the conditions of transformation to increase the efficiency of transformation.It had the optimal rate of shooting when the concentration of 6-BA was 1.7 mgL-1.The optimal selection concentration of Glufosinate-ammonium for shoot initiation was 3.5 mgL-1Transformed plants containing pCAMBIA3300-Bt was obtained and 19 positive plants from T1generation were tested by PCR.Real-time PCR was used to investigate the transcription level of Bt gene in T1transgenic plants，it confirmed the integration of Bt genes into the genome of soybean.

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Last Update: 2014-10-03