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Isolation of Acidic Subunits and Basic Subunits from Glycinin using Isoelectric Point Precipitation(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2009年01期
Page:
136-139
Research Field:
Publishing date:

Info

Title:
Isolation of Acidic Subunits and Basic Subunits from Glycinin using Isoelectric Point Precipitation
Author(s):
ZHENG Shu-gui1; 2; DONG Wei-guo1; SUN Ze-wei1; QIN Gui-xin1
1College of Animal Science and Technology，Jilin Agricultural University，Changchun 130118，Jilin；
2College of Animal Husbandry and Veterinary Medicine，Shenyang Agricultural University，Shenyang 110161，Liaoning，China
Keywords:
Isolation; Acidic subunits; Basic subunits; Purity; Productive rate
PACS:
TS214
DOI:
10.11861/j.issn.1000-9841.2009.01.0136
Abstract:
A novel isoelectric point precipitation method of isolation of Glycinin acidic and basic subunit was developed.In this approach，two kinds of subunits was isolated according to the difference of isoelectric point of the subunits after disulfide bonds between acidic and basic subunits was cut off using β-mercaptoethanol.The purity of acidic and basic subunits prepared by this approach were 92.69% and 80.76%，and productive rate reached 31.40%，41.64%，repectively.So the approach we developed was a simple，efficient and cheap method for isolation of Glycinin acidic and basic subunits.

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Last Update: 2014-10-03