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Effects of Hirsutella Minnesotensis Metabolites on Soybean Cyst Nematode Juvenile(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2009年01期
Page:
118-121,129
Research Field:
Publishing date:

Info

Title:
Effects of Hirsutella Minnesotensis Metabolites on Soybean Cyst Nematode Juvenile
Author(s):
QIAN Hong-li1; 2; XU Yan-li2; SUN Yu-qiu2; LI Chun-jie2
1College of Life Sciences，Northeast Forestry University，Harbin 150040，Heilongjiang；
2 Northeast Institute of Geography and Agroecology，Harbin 150081，Heilongjiang，China
Keywords:
Hirsutella minnesotensis; Metabolites; Soybean cyst nematode; Second stage juveniles; Tendency
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.01.0118
Abstract:
As an fungal endoparasites of soybean cyst nematodes(SCN）juvenile，Hirsutella minnesotensis was a potential biological control agent.To discuss the mechanism of Hirsutella minnesotensis on SCN，the effect of Hirsutella minnesotensis 1-10 metabolites at the original and different dilute solution on second stage juveniles(J2)mortality and its effect on the attraction of J2 to soybean root diffusate or soybean root on agar plate was investigated.It was deduced that both the original and different dilute solution of 1-10 metabolites could significantly restrain the activity of J2，and that the effect of the metabolites at the original，5，10，20 and 50 on the mortality of J2 were 91%，75%，50%，36% and 31% after 24 hours.The difference between these treatments and water control were significant，and as the time went，the mortalities of J2 grew.J2 suspension was inoculated on the centre of a circle of water agar plate and the filter paper strips soaking up with root diffusate or the metabolites were place at a 3 cm distance around the centre.The results showed that J2 were repelled by 1-10 metabolites according the lowest distribution rates of J2 on 0-1 cm area nearby filter paper strips.In the same area，it showed that J2 were attracted toward the root diffusate according the highest distribution rates.Further more，J2 were significantly lower in the area treated by mixture of root diffusate and 1-10 metabolites than the sterile water(0.05).The same result was obtained by using soybean roots or the roots dipped with 1-10 metabolites（P<0.05).It is deduced that 1-10 metabolites can reduce the tendency of soybean cyst nematode toward soybean roots.

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Last Update: 2014-10-03