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Response of Enzyme Activity and Secondary Metabolites of Different Soybean Genotypes to Aphis glycines Matsmura Invasion(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2009年01期
Page:
103-107
Research Field:
Publishing date:

Info

Title:
Response of Enzyme Activity and Secondary Metabolites of Different Soybean Genotypes to Aphis glycines Matsmura Invasion
Author(s):
JIANG Yi-na; WANG Biao; WU Tian-long
School of Agriculture and Biology，Shanghai Jiao Tong University，Shanghai 200240，China
Keywords:
Soybean aphid; POD; PPO; PAL; Secondary metabolites
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.01.0103
Abstract:
In this experiment we examined two soybean varieties including P746 with resistance to aphid and Jiaoda 02-89 with susceptivity to aphid to study the mechanism of the aphid resistance through the analysis of the enzymatic activity of Peroxidase(POD)，Polyphenol Oxidase(PPO)，Phenylalanine Ammonia-lyase(PAL) and the content of secondary metabolites.The results showed that the enzyme activity of PPO and POD expressed at higher level in each variety after infected by aphid，but there was no obvious difference between resistance and susceptible materials.The PAL enzyme activity was different between resistance and susceptible materials，it was higher in the resistance material than that of susceptible one before aphid infected.The activity expressed at higher level after induced by aphid，but the change in the resistance materials was not obvious.Besides，we analyzed the changes of the secondary metabolites infected by soybean aphids using HPLC methods，the result indicated that the content of some secondary metabolites in leaf tissue such as amino acid，flavonoid，alkaloid，phenol increased after infected by aphid，and the changes between the two materials were obvious too，which demonstrate that the aphid resistance of soybean might rely on these substances.

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Last Update: 2014-10-03