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Cloning and Sequence Analysis of a C2H2 type Zinc Finger Protein Gene from Glycine soja(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2009年01期
Page:
21-25
Research Field:
Publishing date:

Info

Title:
Cloning and Sequence Analysis of a C2H2 type Zinc Finger Protein Gene from Glycine soja
Author(s):
BAI Jing1ZHANG Bi-xian2LI Xin-ling1XU Xiang-ling1
1 Life Science and Technology Institute,Harbin Normal University,Harbin 150025,Heilongjiang;
2 Post doctorial Station of Heilongjiang Academy of Agricultural Science,Harbin 150080,Heilongjiang,China
Keywords:
Wild soybeanC2H2 type zinc finger proteinGene cloning
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.01.0021
Abstract:
Specific primers were designed according to the soybean SCOF-1 of CDs from the GenBank,C2H2-type Zinc finger protein gene containing two zinc finger,named GjC2H2 was cloned through the PCR and RT-PCR from the wild soybean 01-197,and the GenBank accession number was FJ172776.Homogenesis and phylogeny analysis of nucleotide,amino acid sequence showed that there was no intron in the gene,the length of the gene was 702 bps,molecular weight was 25.13 KD,and the gene and other zinc finger protein gene had a high homology.It is presumed that the gene GjC2H2 is related with stress tolerance in plants.

References:

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Last Update: 2014-09-29