|Table of Contents|

Cloning and Prokaryotic Expression Vector Construction of Gly m Bd 30K Gene from Soybean(Glycine max)(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2009年01期
Page:
11-15
Research Field:
Publishing date:

Info

Title:
Cloning and Prokaryotic Expression Vector Construction of Gly m Bd 30K Gene from Soybean(Glycine max)
Author(s):
WU Yu-lan; LIU Zhi-gang
Allergy and Immunology Institute，Shenzhen University，Shenzhen 518060，Guangdong,China
Keywords:
Soybean(Glycine max); Allergen; Gly m Bd 30K; Cloning; Prokaryotic Expression Vector
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.01.0011
Abstract:
The RT-PCR was applied to clone the full- length allergen genes from soybean and the sequences were analyzed.The specific primers were designed.The ORF of Gly m Bd 30K of soybean was subcloned into the expression vector pET 28a.Results showed that the open reading frame（ORF）of cloned cDNA contained 1 140 bp and encoded 379 amino acids，and the estimated molecular mass of the encoded protein was 42 758 with pI 5.08.Sequence analysis showed that this clone shared high identities with Gly m Bd 30K from soybean.The deduced protein was therefore regarded as the major allergen of Gly m Bd 30K of soybean（GenBank database entry No.EU883600）.Cloning and prokaryotic Expression Vector Construction of Gly m Bd 30K gene from soybean，which will be used as base for the expression and identification of allergen protein.

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Last Update: 2014-09-29