|Table of Contents|

Design of Tandem Genes Cluster and Expression Vectors for Soybean Isoflavone Biosynthesis(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2009年01期
Page:
7-10,20
Research Field:
Publishing date:

Info

Title:
Design of Tandem Genes Cluster and Expression Vectors for Soybean Isoflavone Biosynthesis
Author(s):
XIA Xun-li; YANG Guang-xiao; HE Guang-yuan
China UK HUST RRes Genetics Engineering and Genomics Joint Laboratory，Huazhong University of Science and Technology，Wuhan 430074，Hubei，China
Keywords:
Soybean isoflavones; Biosynthesis; Tandem genes cluster; Expression vector
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2009.01.0007
Abstract:
Plant isoflavones is a sort of important secondary metabolites with signification in plant physiological function and healthiness for human being.There are three regulating enzymes in plant isoflavones biosynthesis pathway：Chalcone synthase(CHS)，Chalcone isomerase(CHI）and Isoflavone synthase(IFS).In this article，we cloned the cDNAs of CHS，CHIⅡ，and IFS using RT-PCR method；then assembled these three genes to make a tandem genes cluster-CHS-CHI Ⅱ-IFS(we named it as rIFS)；finally we constructed the E coli.expression vector of pET-rIFS and researched its expression.All these made the groundwork for further research on soybean isoflavone biosynthesis and isoflavone engineering.

References:

[1]黄芸，秦民坚，余国英．异黄酮类化合物在植物界的分布及药理作用[J]．中国野生植物资源，2001，20(1)：5-7．（Huang Y，Qin M J，Yu G Y.Distributing of isoflavones in plants and their pharmacologicals functions[J].Chinese Wild Plant Resourses，2001，20(1)：5-7.）

[2]杨茂区，陈伟，冯磊．大豆异黄酮的生理功能研究进展[J]．大豆科学，2006，25(3)：320-324.（Yang M Q，Chen W，Feng L.Research progress of biological functions of soybean isoflavone[J].Soybean Science，2006，25(3)：320-324.）
[3] Yu O，Jung W，Shi J，et al.Production of the isoflavones genistein and daidzein in non-legume dicot and monocot tissues[J].Plant Physiology，2000，124，781-793.
[4] Liu C J，Jack W B，Christopher L S，et al.Bottlenecks for metabolic engineering of isoflavone glycoconjugates in Arabidopsis[J].Proceeding of the National Academy of Sciences，2002，99(22)：14578-14583.
[5] Brenda W S.Flavonoid biosynthesis.A colorful model for genetics，biochemistry，cell biology，and biotechnology[J].Plant Physiology，2001，126：485-493.
[6] Ralston L，Subramanian S，Matsuno M，et al.Partial reconstruction of flavonoid and isoflavonoid biosynthesis in yeast using soybean type I and type II Chalcone isomerases[J].Plant Physiology，2005，137(4)：1375-1388.
[7] Akada S，Kung S D，Dube S K.Nucleotide sequence of a soybean chalcone synthase gene with a possible role in ultraviolet-B sensitivity，Gmchs 6[J].Plant Physiology，1993，102(2)：699-701.
[8] Jung W，Yu Q，Lau S M C，et al.Identification and expression of isoflavone synthase，the key enzyme for biosynthesis of isoflavones in legumes[J].Nature Biotechnology，2000，18：208-212.
[9]陆云华，马立新，蒋思婧．一种通用高效的复杂载体构建的新方法[J]．遗传，2006，28（2）：212-218．（Lu Y H，Ma L X，Jiang S J.A universal high throughput novel method of constructing the vectors[J].Hereditas，2006，28(2)：212-218.）

Memo

Memo:
-
Last Update: 2014-09-29