|Table of Contents|

Extraction and Characterization of Soybean Trypsin Inhibitor(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2008年06期
Page:
1085-1088
Research Field:
Publishing date:

Info

Title:
Extraction and Characterization of Soybean Trypsin Inhibitor
Author(s):
TIAN Jun1; WEI Zhi-kui2; ZHOU Xian-wan2
1Higher Education Press，Beijing 100029；
2College of Life Sciences，Peking University，Beijing 100871
Keywords:
Soybean; Trypsin inhibitor; Isoelectric focusing electrophoresis
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.06.1085
Abstract:
Protease inhibitor is a kind of polypeptides or proteins which has inhibition effect on protease and its highest inhibition activity was observed in the seed of soybean.The soybean trypsin inhibitors were extracted and purified by DEAE-Sepharose 4B FF and SP-Sepharose 4B FF column，and the activities of each obtained component were measured.Meanwhile，SDS-PAGE and isoelectric focusing electrophoresis were also used to determine the relative molecular weight and isoelectric point.Result showed the component with the highest inhibition activity has the specific inhibition activity of 15 000 U.mg-1，the Mr?of 21 000 and the isoelectric point of 4.55.

References:

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Last Update: 2014-10-06