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Identification of A Strain with High Ability of Producing Glutaminase and Study of Enzymatic Characterization(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2008年06期
Page:
1037-1040
Research Field:
Publishing date:

Info

Title:
Identification of A Strain with High Ability of Producing Glutaminase and Study of Enzymatic Characterization
Author(s):
HAN Ming-hai; ZHAO Juan-juan; HUA Sheng-long; YANG Ye; TANG Jiao; YAO Ting
Department of Biology，Huaiying Teachers College，Huaian 223300，Jiangsu，China
Keywords:
Glutaminase; Soy sauce; Citrobacter sp.; Identification
PACS:
TQ925
DOI:
10.11861/j.issn.1000-9841.2008.06.1037
Abstract:
In the paper，a strain with high ability of producing glutaminase was identified and enzymatic characterization of glutaminase was studied.Accroding to morphology，physiological and biochemical characteristics，it can be roughly concluded that the strain belongs to Citrobacter.The relationship between pH，temperature，NaCl，glutamic acid and glutaminase was studied.The optimum temperature and pH of glutaminase from the strain was 50℃ and 6.2，respectively.When pH was 6.2 and temperature below 40℃，the enzyme showed high thermal stability.High concentration of NaCl didn’t not have negative influence on enzyme activity and glutamic acid didn’t pose considerable inhibition to glutaminase.

References:

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Last Update: 2014-10-06