|Table of Contents|

Effect of Soybean Isoflavones and Saponins on Proliferation and Apoptosis of Human Colon Carcinoma Cell Line Ht-29(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2008年06期
Page:
1028-1031
Research Field:
Publishing date:

Info

Title:
Effect of Soybean Isoflavones and Saponins on Proliferation and Apoptosis of Human Colon Carcinoma Cell Line Ht-29
Author(s):
JIN Mei-hua; XU Hui-xian; JIN Hua; QUAN Ji-shu; YIN Xue-zhe
Medical College of Yanbian University，Yanji 133000，Jilin，China
Keywords:
Soybean; Isoflavones; Saponins; Colon cancer; Cell cycle; Apoptosis
PACS:
B282.7
DOI:
10.11861/j.issn.1000-9841.2008.06.1028
Abstract:
Soybean isoflavones and saponins have been observed to limit many kind of tumor cell proliferation by inducing apoptosis.Soybean isoflavones and saponins were extracted from soybean hypocotyl，then investigated for the inhibition on proliferation and apoptosis of human colon carcinoma HT-29 cell.MTT assay was used to examine the proliferation of HT-29，flow cytometry was used for analysis of cell cycle distribution，and immunohistochemical method was used for the determination of bax，bcl-2 and p53 expression.The results showed that soybean isoflavones and saponins inhibited the proliferation of colon carcinoma cell in a time- and concentration-dependent manner，induced apoptosis and lead to G2/M arrest.The apoptotic ratio of treatment group was significantly lower than that of control group，the expression of bax increased and bcl-2 decreased significantly.It is suggested that soybean isoflavones and saponins could exert an anti-colon cancer effect via cell cycle arrest and induction of apoptosis.

References:

[1] Wang H J，Murphy P A.Isoflavone contents in commercial soybean foods[J].Journal of Agricultural and Food Chemistry，1994，42(8):1666-1673.

[2] Branham W S，Dial S L，Moland C L，et al.Phytoestrogens and mycoestrogens bind to the rat uterine estrogen receptor[J].The Journal of Nutrition，2002，132(4):658-664.
[3]Vedavanam K，Srijayanta S，O′Reilly J，et al.Antioxidant action and potential antidiabetic properties of an isoflavonoid-containing soybean phytochemical extract(SPE)[J].Phytotherapy Research，1999，13(7):601-608.
[4]Kerry N，Abbey M.The isoflavone genistein inhibits copper and peroxyl radical mediated low density lipoprotein oxidation in vitro[J].Atherosclerosis，1998，140(2):341-347.
?[5]Konoshima T.Anti-tumor-promoting activities or triterpenoid glycosides；cancer chemoprevention by saponins[J].Advances in Experimental Medicine and Biology，1996，404:87-100.
[6] Rodrigues H G，Diniz Y S，Faine L A，et al.Antioxidant effect of saponin： potential action of a soybean flavonoid on glucose tolerance and risk factors for atherosclerosis[J].International Journal of Food Sciences and Nutrition，2005，56(2):79-85.
[7]Yoshikoshi M，Yoshiki Y，Okubo K，et al.Prevention of hydrogen peroxide damage by soybean saponins to mouse fibroblasts[J].Planta Medica，1996，62(3):252-255.
[8]Hayashi K，Hayashi H，Hiraoka N，et al.Inhibitory activity of soyasaponin II on virus replication in vitro[J].Planta Medica，1997，63(2):102-105.
[9] Kinjo J，Imagire M，Udayama M，et al.Structure-hepatoprotective relationships study of soyasaponins I-IV having soyasapogenol B as aglycone[J].Planta Medica，1998，64(3):233-236.
[10]尹学哲，许惠仙，金花.大豆异黄酮和皂苷对糖尿病大鼠脂蛋白氧化修饰的抑制作用[J].大豆科学，2008，27(1):170-172.（Yin X Z，Xu H X，Jin H.Inhibition of soybean isoflavones and saponins on oxidation of lipoproteins in diabetic rats[J].Soybean Science，2008，27(1)：170-172.)
[11]Melet A，Song K，Bucur O，et al.Apoptotic pathways in tumor progression and therapy[J].Advances in Experimental Medicine and Biology，2008，615:47-79.
[12]Tan T T，White E.Therapeutic targeting of death pathways in cancer：mechanisms for activating cell death in cancer cells[J].Advances in Experimental Medicine and Biology，2008，615:81-104.
[13]Korsmeyer S J.Bcl 2 gene family and regulation of programmed cell death[J].Cancer Research，1999，59(7s):1693s-1700s.
[14]Lu Q L，Poulsom R，Wong L，et al.Bcl-2 expression in adult and embryonic non-haematopoietic tissues[J].Journal of Pathology，1993，169(4):431-437.
[15]姚保泰，吴敏，王博.盐酸小檗碱抑制实验性大鼠胃癌前病变细胞bcl-2表达作用的研究[J].中国中医药杂志，2005，20(3):186-187.(Yao B T，Wu M，Wang B.Experimental research on repairing myocardial cells of viral myocarditis by study on inhibiting action of hydrochloric berberine on expression of bcl-2 in cells of precancerous lesion in experimental rat model[J].China Journal of Traditional Chinese Medicine and Pharmacy，2005，20(3):186-187.)

Memo

Memo:
-
Last Update: 2014-10-06