|Table of Contents|

A New Method for Detecting Bean Pod Mottle Virus by GICA-RT-PCR(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2008年06期
Page:
1019-1023
Research Field:
Publishing date:

Info

Title:
A New Method for Detecting Bean Pod Mottle Virus by GICA-RT-PCR
Author(s):
ZHANG Xiao-lei1; TAN Gen-jia1; WEI Mei-sheng2; ZHANG Yong-jiang2; LI Gui-fen2
GICA；Immunostrip；BPMV；GICA-RT-PCR
Keywords:
GICA; Immunostrip; BPMV; GICA-RT-PCR
PACS:
S435.6
DOI:
10.11861/j.issn.1000-9841.2008.06.1019
Abstract:
1Plant Protection School，Anhui Agricultural University，Hefei 230036,Anhui；2Institute of Animal and Plant Quarantine，Chinese Academy of Inspection and Quarantine，Beijing 100029，ChinaAbstract： Bean pod mottle virus(BPMV)is a quarantine pest for China.A high sensitive and rapid gold immunochromatography assay-RT-PCR(GICA-RT-PCR )method has been developed for the detection of BPMV- infected soybean seed.This method takes the advantage of gold immunochromatography assay(GICA)which captures the virus first on test line，then the immunocaptured virus will be used for direct RT-PCR to amplify the target nucleic acid，eliminating the need for traditional total RNA extraction methods and reducing the number of steps involved.The sensitivity of the GICA-RT-PCR for purified virus is 0.01 μg.mL-1.BPMV-infected soybean leaves and soybean seed coat diluted to 10-4can be detected by GICA-RT-PCR.GICA-RT-PCR is 20 times，10 times and 100 times more sensitive than GICA for detecting purified BPMV，BPMV-infected soybean leaves and soybean seed coat，respectively.GICA-RT-PCR is a simple and sensitive tool for confirmation of GICA results.

References:

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Last Update: 2014-10-06