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Isolation of a Bacteria from Wild Soybean Rhizosphere Soil and Its Function to Control Soybean Diseases(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2008年06期
Page:
1007-1009,1014
Research Field:
Publishing date:

Info

Title:
Isolation of a Bacteria from Wild Soybean Rhizosphere Soil and Its Function to Control Soybean Diseases
Author(s):
LI Tie1; LIU Zhao-jun1; LIU Li-yan1; ZHANG Li-li1; CHENG Yu1; MA Qi-hui2
1.Biotechnology Research Institute，Heilongjiang Academy of Agricultural Sciences(HAAS)，Harbin 150086,Heilongjiang；
2.Infornation Institute of HAAS，Harbin 150086，Heilongjiang，China
Keywords:
Glycine soja; Antagonism; Soybean continuous cropping
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.06.1007
Abstract:
Through screening edaphon resisting soybean root rot，one bacterial strain was isolated from wild soybean rhizosphere in medium with phthalate as only carbonaceous organic compound.It could digest phthalate and was named HK26-21.Through plate inoculation and 16s rDNA sequence amplification，it was showed that HK26-21 was Bacillus according to physiology-biochemistry characteristics and the similarity of 16s rDNA analysis.Through single factor test，the optimal digesting conditions were：temperature 25 ℃，pH 7.0.Digesting rate had positive correlation with the amount of inoculums.Test of colony standoff in plate proved the bacterium could resist soybean pathogenic fungi(Fusarium oxysporum and Rhizoctonia solani).It was considered that the isolated bacterium from wild soybean rhizosphere could have biological control efficacy against pathogens of soybean root rot.

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Last Update: 2014-10-06