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Molecular Cloning and Sequence Analysis of Δ′-pyrroline-5-carboxylate Synthetase（P5CS）Gene in Wild Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2008年06期
Page:
915-920
Research Field:
Publishing date:

Info

Title:
Molecular Cloning and Sequence Analysis of Δ′-pyrroline-5-carboxylate Synthetase（P5CS）Gene in Wild Soybean
Author(s):
ZHANG Chun-bao1; 2; ZHAO Hong-kun1; LI Qi-yun1; LIU Xiao-dong1; SHEN Bo1; DONG Ying-shan1; 2
1 Biotechnology Research Center，Jilin Academy of Agricultural Sciences，Changchun 130033，Jilin；
2College of Life Science，Northeast Normal University，Changchun 130024，Jilin，China
Keywords:
Wild soybean; Δ′-pyrroline-5-carboxylate synthetase; Salt resistance
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.06.0915
Abstract:
GsP5CS，homologous with the GmP5CS gene encoding a Δ′-pyrroline -5-carboxylate synthetase was cloned from wild soybean（Glycine soja）by RT-PCR，with primers designed according to the sequence of GmP5CS.The largest open reading frame of GsP5CS gene has 2148bp in length and encoded a protein of 716 amino acid residues.The estimated molecular weight and isoelectric points of the putative protein was 77.9 kDa and 6.21，respectively.Hydropathy plot analysis indicated that the GsP5CS was hydrophilic.Blast result analysis showed that GsP5CS，GmP5CS，MsP5CS，MtP5CS，AtP5CS，BnP5CS，OsP5CS，TaP5CS shared a cluster.The phylogenetic tree shows that the relationship based on the homology is consistent with the morphological and taxonomic results.

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Last Update: 2014-10-04