|Table of Contents|

Comparison on Effect of Improved Chelex-100 Method and CTAB Method for Transgenic Roundup Ready Soybean Detection(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2008年05期
Page:
898-901
Research Field:
Publishing date:

Info

Title:
Comparison on Effect of Improved Chelex-100 Method and CTAB Method for Transgenic Roundup Ready Soybean Detection
Author(s):
WANG Yong1; LAN Qing-kuo1; ZHANG Li2; ZHAO Xin1; ZHU Zhu1; CHENG Yi1
1Central Lab of Tianjin Academy of Agricultural Sciences,Tianjin 300381；
2Tianjin Institution of Animal Husbandry,Tianjin 300112，China
Keywords:
Chelex-100 method; CTAB method; DNA; transgenic detection
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.05.0898
Abstract:
Improved Chelex-100 method and conventional CTAB method were applied to extract total DNA from transgenic roundup ready soybean.Both improved chelex-100 method and CTAB method were compared in this study，and quantity and purity of the extracted DNA were evaluated by PCR amplification result of the endogenous gene (lectin) and foreign specific sequence (CaMV35S、nos and Cp4-epsps).Despite the lower quality of DNA,the Improved Chelex-100 method can quickly extract the DNA from soybean within 1 hour.The DNA can be directly applied to the following step-PCR amplification as DNA template.Therefore,Chelex-100 is economic,simple and rapid,which can be replaced CTAB for transgenic DNA detection.

References:

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Last Update: 2014-10-06