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Enzymatic Hydrolysis Technology and Quality Standards of Soybean Minor Peptides(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2008年05期
Page:
863-865，873
Research Field:
Publishing date:

Info

Title:
Enzymatic Hydrolysis Technology and Quality Standards of Soybean Minor Peptides
Author(s):
GE Sheng-dong1; LI Mao-hui2; GAO Shu-jie3; LU Gui-hua4; LIU Ying-ping5
1 Deptarment of Cellular Biology and Genetics of Shenyang Medical College,Shenyang 110034；
2 Department of Preventive Medicine Shenyang Medical College,Shenyang 110034；
3Deptarment of Sport Biochemistry of Shenyang Sport College，Shenyang 110031；
4Disease Control Center of Liaoning Province,Shenyang 110001；
5Tianling Pharmaceutical Company of Shenyang,Shenyang 110003，Liaoning，China
Keywords:
Soybean minor peptides;  Crude complex trypsinase;  Neutral proteinase; Peptide yield; Qualitative standards
PACS:
Q814
DOI:
10.11861/j.issn.1000-9841.2008.05.0863
Abstract:
In this study，the new extraction technique of soybean bioactive minor peptides from defatted soy flakes hydrolysized by crude complex trypsinase and neutral proteinase was set up．Optimal condition of pretreatment and hydrolyzing by crude .g-1，temperature 55℃，pH 8.5，time 6 h， under the condition of 5.0% substrate concentration.And the optimum condition of neutral proteinase hydrolysis was given by enzyme quantity 8 500 U.g-1，pH 7.5；temperature 45℃ ；treatment time 2 h．Under this condition，the ratio of soybean minor peptides reached 73.98 %． And the quality standards of soybean bioactive minor peptides were also established.

References:

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Last Update: 2014-10-06