|Table of Contents|

Inhibition of Adhesion of in Vitro Hydrolysate of β-conglycinin to Caco-2 Cell by Enteropathogens(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2008年05期
Page:
845-849
Research Field:
Publishing date:

Info

Title:
Inhibition of Adhesion of in Vitro Hydrolysate of β-conglycinin to Caco-2 Cell by Enteropathogens
Author(s):
WANG Jin; YANG Bai-chong; LU Ying; GUO Shun-tang
College of Food Science and Nutritional Engineering，China Agriculture University，Beijing 100083，China
Keywords:
Yellow soybean; Black soybean; Hydrolysate of β-conglycinin; Inhibition of adhesion; Caco-2 cell
PACS:
Q556
DOI:
10.11861/j.issn.1000-9841.2008.05.0845
Abstract:
The materials of this study were β-conglycinins extracted from yellow soybean and black soybean，of which we detected the construction of subunits and the content of combining-sugar by sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and periodic acid-Schiff (PAS).To investigate the effect on intestinal microbial community，in vitro experiments were conducted using Caco-2 cell line to confirm that in vitro pepsin and trypsin hydrolysates of β-conglycinin from yellow soybean and black soybean had an antiadhesive effect on human intestinal cells against the enteropathogens (Escherichia coli strains serotype O26，Salmonella typhimurium LT2).The results showed that there was no disparation in both construction of subunits and sugar content of β-conglycinins from the two kinds of soybeans,of which sugar content were 3.5%~3.7%，and their hydrolysates after heat treatment could significantly inhibit the adhesion of Escherichia coli-O26 to Caco-2 cells，while there was no effect against Salmonella typhimurium LT2.Our findings indicated that β-conglycinin probably had an antiadhesive effect on protecting human intestinal cells against the Escherichia coli O26.

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Last Update: 2014-10-06