|Table of Contents|

Extraction of Soybean Oligosaccharides by Microwave and Ultrasonic Technique(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2008年05期
Page:
838-844
Research Field:
Publishing date:

Info

Title:
Extraction of Soybean Oligosaccharides by Microwave and Ultrasonic Technique
Author(s):
LIU Li-yang1JIN Long-guo 2LIU Zhang-Xiong 2HAO Zai-bin 1CHANG Ru-zhen2QIU Li-juan 2
1Life Science College of Northeast Agricultural University,Harbin 150030,Heilongjiang;
2The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI) / Key Laboratory of Germplasm & Biotechnology (MOA),Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,100081 Beijing,China
Keywords:
Soybean oligosaccharidesMicrowaveultrasonicOrthonogal experimentsHPLC
PACS:
S565.1Q532
DOI:
10.11861/j.issn.1000-9841.2008.05.0838
Abstract:
To Investigate the extraction efficiency of soybean oligosaccharides by microwave and ultrasonic and provide experimental evidence for further soybean oligosaccharides extraction.The orthogonal experimental design is used to compare the extraction efficicency of microwave and ultrasonic extraction.The microwave extraction selected orthogonal experimental table L9(34) and the optimization condition was 70%ethanol,the ratio of material to solvent 1∶10,high power,2 minutes.The ultrasonic extraction selected orthogonal experimental table L16(44) and the optimization condition is 75%ethanol,the ratio of material to solvent 1:10,40 KHz,60℃,1.5 hours.HPLC was used to measure the extraction efficiency.The optimization condition selected by standard substance stachyose,raffinose and sucrose were mobile phase acetonitrile:water(60:40),temperature 35℃,velocity of flow 1.0 mL.min-1.The results indicate the efficiency and effect of extraction by microwave are both better than that of extraction by ultrasonicand the microwave extraction method is a simple,easy-to-use and suitable method for soybean oligosaccharides extraction.

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Last Update: 2014-10-06