|Table of Contents|

Determination of Isoflavone Content in Soybean by Reverse Phase High Performance Liquid Chromatography(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2008年05期
Page:
828-832,837
Research Field:
Publishing date:

Info

Title:
Determination of Isoflavone Content in Soybean by Reverse Phase High Performance Liquid Chromatography
Author(s):
LI Wei-xi; LIU Fang; LI Yan; LIU San-cai; ZHU Zhi-hua
The National Key Facilities for Crop Genetic Resources and Improvement，Institute of Crop Science，CAAS，The Supervision and Testing Center for Crop Germplasm Resources，MOA，Beijing 100081，China
Keywords:
RP-HPLC; Daidzin; Genistin; Glycitin; Agricultural standard
PACS:
S565.1Q501
DOI:
10.11861/j.issn.1000-9841.2008.05.0828
Abstract:
Isoflavone is a group of special biofunctional substance.In this paper，a method determining isoflavone including Daidzin，Glycitin and Genistin by Reverse Phase High Performance Liquid Chromatography (RP-HPLC) was investigated and erected.Meanwhile，the isoflavone determination method was analysed as well as evaluated wholly and systematically by statistical technique.The results showed that the linear range of Daidzin，Glycitin and Genistin was 1.25-20.0μg.mL-1，0.625-10.0μg.mL-1 and 1.25-20.0μg.mL-1，respectively；the recovery range was 97.1%-99.5%；the relative deviation of repeatable test was below 5%；the relative standard deviation of Daidzin，Glycitin and Genistin among different labs was 1.15%-3.42%，2.44%-4.84% and 1.43%-3.06%，respectively.It showed from the above analysis that this method possessed good linearity，good quality of accuracy，precision，repeatability in one lab and reproducibility among labs.Therefore，this method was proved to be suitable for erecting and implementing a national agricultural standard.

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Last Update: 2014-10-06