|Table of Contents|

Effect of Different Phosphorus Concentrations on Microbial Communities in Soybean Rhizosphere(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2008年05期
Page:
801-805
Research Field:
Publishing date:

Info

Title:
Effect of Different Phosphorus Concentrations on Microbial Communities in Soybean Rhizosphere
Author(s):
LIU Jun-jie12WANG Guang-hua2JIN Jian2LIU Ju-dong2ZHANG Qiu-ying2LIU Xiao-bing2
1Northeast Agricultural University,Resources and Environmental Sciences College,Harbin 150030;
2Key Lab of Black Soil Ecology,Northeast Institute of Geography and Agro-ecology,Chinese Academy of Science,Harbin 150081,Heilongjiang,China
Keywords:
Bactarial communityBlack soilFungal communityPCR-DGGEPhosphorus
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.05.0801
Abstract:
Microbial community in the rhizosphere play an important role in soil nutrient availability and plant growth.Different concentrations of phosphorus availability change the root exudates and root architecture;however,the effect on microbial community in the rhizophere is unclear.In this study,a pot experiment was conducted to investigate the effects of three phosphorus concentrations on soybean rhizosphere microbial communities at three sampling times (V3,R1 and R3).The bacterial and fungal communities were analyzed by PCR-DGGE (polymerase chain reaction-degenerate gradient gel electrophoresis) targeting a partial 16S rDNA and the internal transcribed space (ITS) region,respectively.DGGE banding patterns were used for principal component analysis (PCA).Results showed that both bacterial and fungal communities shifted with growth stages,suggesting that the growth stage play a more important role in determination of soybean rhizosphere microbial community than different phosphorus concentration treatments.Furthermore,the impact of different phosphorus treatments on microbial community change was only detected at soybean early growth stages,i.e.,for fungal community at stage V3 and for bacterial community at stages V3 and R1.No significant difference of microbial community among phosphorus treatments was observed at growth stage R3.

References:

[1] Wissuwa M.How do plants achieve tolerance to phosphorus deficiencySmall causes with big effects[J].Plant Physiology,2003,133(4):1947-1958.

[2]Zhang L M,He L Y,Li J S,et al.Phosphorus nutrient characteristics of diferent maize (Zea mays) inbreds for tolerance to low-P stress[J].Agricultural Sciences in China,2005,4(4):281-287.
[3]潘相文,唐才贤,王光华,等.作物耐低磷适应机制研究进展 [J].吉林农业大学学报,2005,27(4):434-441.( Pan X W,Tang C X,Wang G H,et al.Progress of study on adaptation mechanism of crop tolerance to low phosphorus[J].Journal of Jilin Agricultural University,2005,27(4):434-441.)
[4]林海建,张志明,高世斌,等.玉米耐低磷研究现状及磷高效育种策略的探讨[J].中国农学通报,2008,24(1):181-185.(Lai H J,Zhang Z M,Gao S F,el at.The research actuality of maize(Zea maysL.) tolerance to low phosphorus and the strategy of high phosphorus efficiency breeding[J].Chinese Agricultural Science Bulletin,2008,24(1):181-185.)
[5]严小龙,廖红,戈振扬,等.植物根构型特性与磷吸收效率[J].植物学通报,2000,17(6):511-519.(Yan X L,Liao H,Ge Z Y,et al.Root architectural characteristics and phosphorus acquisition efficiency in plants[J].Chinese Bulletin of Botany,2000,17(6):511-519.)
[6] Asea P E A,Kucey R M N,Stewart J W B.Inoganic phosphate solublization by two Penicillium species in solution culture and soil[J].Soil Biology and Biochemistry,1988,20:459-464.
[7]丁洪,郭庆元,李志玉.磷对大豆品种产量和品质的影响[J].中国油料作物学报,1998,20 (2):67-70.( Ding H,Guo Q Y,Li Z Y,el at.Effect of phosphorus on grain yield and quality in soybean cultivars [J].Chinese Journal of Oil Crop Sciences,1998,20(2):67-70.)
[8]王美丽,严小龙.大豆根形态和根系分泌物特性与磷效率[J].华南农业大学学报,2001,22(3):1-4.( Wang M L,Yan X L.Characteristics on root morphology and root exudation of soybean in relation on phosphorus efficiency[J].Journal of South China Agricultural University,2001,22(3):1-4.)
[9]金剑,王光华,刘晓冰,等.不同施磷量对大豆苗期根系形态性状的影响[J].大豆科学,2006,25(4):360-364.( Jin J,Wang G H,Liu X B,et al.Effect of different phosphorus regimes on root morphological characteristics of soybean seedling[J].Soybean Science,2006,25(4):360-364.)
[10]朱丽霞,章家恩,刘文高.根系分泌物与根际微生物相互作用研究综述[J].生态环境,2003,12(1):102-105.( Zhu L X,Zhang J E,Liu W G.Review of studies on interactions between root exudates and rhizopheric microorganisms[J].Ecology and Environment,2003,12(1):102-105.)
[11] Zhou J,Bruns M A,Tiedje J M.DNA recovery from soils of diverse composition [J].Applied and Environmental Microbiology,1996,62:316-312.
[12]Watanabe T,Asakawa S,Nakamur A,et al.DGGE method for analyzing 16SrDNA of methanogenic archaeal community in paddy field soil [J].FEMS Macrobiology Letters,2004,23(2):153-163.
[13]王光华,刘俊杰,齐晓宁,等.Biolog和 PCR-DGGE技术解析施肥对德惠黑土细菌群落结构和功能的影响[J].生态学报,2008,28(1):220-226.(Wang G H,Liu J J,Qi X N et al.Effects of fertilization on bacterial community structure and function in a black soil of Dehui region estimated by Biolog and PCR-DGGE methods [J].Acta Ecologica Sinica,2008,28(1):220-226.)
[14] Gardes M Bruns,T D.ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts [J].Molecular Ecology,1993,2 :113-118.
[15]Bastias B A,Xu Z H,Cairney J W G.In fluence of long-term repeated prescribed burning on mycelial communities of ectomycorrhizal fungi [J].New Phytologist,2006,172 :149-158.
[16]Yang C H,Crowley D E.Rhizosphere microbial community structure in relation to root location and plant iron nutrition status [J].Applied and Microbiology,2000,66:345-351.
[17]田中民,李春俭,王晨,等.缺磷白羽扇豆排根与非排根区根尖分泌有机酸的比较[J].植物生理学报,2000,26(4):317-322.(Tian Z M,Li C J,Wang C,et al.Comparative studies on organic acid exudation by tips of proteoid and non proteoid roots of phosphorus deficient white lupin [J].Acta Photophy Siologica Sinica,2000,26(4):317-322.)
[18]吴平,印莉蓱,张力平,等 .植物营养分子生理学[M],北京:科学出版社,2001,103-106.(Wu P,Yin L P,Zhang L P,et al.Molecular physiology of plant nutrition[M].Beijing:Science Press,2001,103-106.)
[19]王聪,刘玉平,李秀辉,等.磷胁迫下不同基因型大豆苗期根系形态及生物量的差异[J].中国农学通报,2005,21(2):155-159.(Wang C,Liu Y P,Li X H,et al.Study on variation in characteristics of phosphorus efficiency among different soybean genotypes at seedling stageunder phosphorus stress [J].Chinese Agricultural Science Bulletin,2005,21(2):155-159.)
[20]王光华,金剑,徐美娜,等.植物、土壤及土壤管理对土壤微生物群落结构的影响[J].生态学杂志,2006,25(5):550-556.(Wang G H,Jin J,Xu M N,et al.Effect of plant,soil and soil management on soil microbial community diversity[J].Chinese Journal of Ecology,2006,25(5):550-556.)
[21]Picard C,De Cello F,Venture M,et al.Frequencey and biodiversity of 2,4-diacetylphloroglucinol producing bacteria isolated from the maize rhizosphere at different stages of plant growth[J],Applied and Environmental Microbiology,2000,66 :948-955.
[22]Duineveld B M,Kowalchuk G A,Keijzer A,et al.Analysis of bacterial communities in the rhizosphere of chrysanthemum via denaturing gradient gel electrophoresis of PCR-amplified 16S sRNA as well as DNA framents coding for 16 sRNA[J].Applied and Environmental Microbiology,2001,67:172-178.

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Last Update: 2014-10-06