|Table of Contents|

Optimization of Somatic Embryo Induction and Genetic Analysis of Offspring(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2008年05期
Page:
751-754,759
Research Field:
Publishing date:

Info

Title:
Optimization of Somatic Embryo Induction and Genetic Analysis of Offspring
Author(s):
SONG Xiao-hui1; LI Dong-mei1; DUAN Ying-ying1; HAN Ying-peng1; LI Chun-guang2; LI Wen-bin1
1Soybean Research Institute,Key Laboratory of Soybean Biology of Chinese Education Ministry，Northeast Agricultural University，Harbin 150030;
2Rice Research Institute of Land Reclamation Academy of Heilongjiang Province，Jiamusi 154025，Heilongjiang,China
Keywords:
Soybean regeneration; Immature cotyledon; Somatic embryogenesis induction;  Genetic ananlysis
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.05.0751
Abstract:
With Peking immature cotyledon as explants，key factors that affecting somatic embryogenesis were evaluated.The results showed that pH = 7.0，explant size of 2-3 mm and dark culture conditions were beneficial for the occurrence of somatic embryogenesis，which was proved efficient for somatic embryogenesis.One hundred and fifty F5：6recombinant inbred lines derived from Peking×Keburi was cultured with inducing medium for embryogenesis.The results showed that frequency distribution was discrete，suggesting that embryogenesis was controlled by few genes.Meanwhile，the impact of Keburi on embryogenesis efficiency was higher that that of Peking.

References:

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Last Update: 2014-10-06