|Table of Contents|

Enzymes Hydrolyzing in Vitro Anti-Nutritional Factors in Soybean Meal(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2008年04期
Page:
663-668
Research Field:
Publishing date:

Info

Title:
Enzymes Hydrolyzing in Vitro Anti-Nutritional Factors in Soybean Meal
Author(s):
CHEN Nai-song; YANG Zhi-gang; CUI Wei-dong; ZHOU Jie; MA Jian-zhong; WANG Zhan-fu; ZHENG Jian-wei
College of Aqua-life Science and Technology，Shanghai Fisheries University，Shanghai 200090，China
Keywords:
Enzyme preparation; Degradation; Soybean meal; Anti-nutritional factors
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.04.0663
Abstract:
In vitro experiments with several enzyme preparations were conducted to evaluate the effects of several kinds of single enzyme and compound enzyme and Ca2+on hydrolyzation of relative anti-nutritional factors (ANFs) in soybean meal (SBM).Four single enzymes (phytase，pectase，cellulase，xylanase) and three enzyme compounds (SB-including α-galactosidase and xylanase，C3 including phytase，pectase and cellulase，C5 including phytase，pectase，cellulase，xylanase and SB) were employed.A beforehand test showed that the suitable hydrolytical parameter for these enzymes was similarly 50% for moisture，4.8-5.0 for pH，50℃ for incubation temperature，45 minutes for incubation time.With the parameter and addition of 10.5 U phytase in 1 g SBM，the degradation rate of phytate reached 69.63%.The degradation rates of pectin and cellulose were 45.93% and 66.21% with adding 16 U pectase and 40 U cellulase in 1 g SBM respectively，and with adding 80 U xylanase and 0.7 U a-galactosidase in 1 g SBM，the release rates of reducing sugar increased to 84.77% and 65.19% respectively.With 0.2% CaCl2 supplementation in SBM，the degradation rate of phytate reached 71.09%，slightly higher than 69.63% for the control and the release rates of reducing sugar increased to 88.68% and 65.23% respectively，but the degradation rates of pectin and cellulose increased significantly to 90.01% and 70.43%.Synergistic effects on phytate，pectin and cellulose degradation as well as release of reducing sugars were observed when either C3 or C5 was used.This research indicated that multi-enzyme preparation with moderate CaCl2 supplementation was feasible to hydrolyze in vitro specific ANFs in SBM.

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Last Update: 2014-10-07