|Table of Contents|

Study on Fractionation of Soybean Protein with Rennase from Mucor Pusillus(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2008年04期
Page:
654-658
Research Field:
Publishing date:

Info

Title:
Study on Fractionation of Soybean Protein with Rennase from Mucor Pusillus
Author(s):
LIU ZhaoGUO Shun-tangLi Ying
College of Food Science and Nutrition Engineering,China Agricultural University,Beijing 100083,China
Keywords:
Soybean proteinGlycininβ-conglycininRennaseMucor pusillusFractionation
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.04.0645
Abstract:
The major storage proteins in soybeans are glycinin and β-conglycinin,which play important roles in food and nonfood soy protein products due to their different functional properties.In conventional methods,reductants which were bad for consumer health were commonly used to separate soybean glycinin and β-conglycinin.In this study,Rennase made from Mucor pusillus was found to have different characteristics in clotting milk proteins and soy proteins by micro-Kjeldahl method.Rennase was found to clot 11S fraction particularly by further researches.The separation was proved not due to the pH value decreasing caused by the rennase simply and may be relevant to the special clotting effect of rennase,and this characteristic could be used to separating 7S and 11S fractions.

References:

[1] Derbyshire E,Wright D B,Bloulter L.Storage protein of legume seeds [J].Phytochemistry,1976,15:3-24.

[2] Kitamura K,Takagi T,Shibasaki K.Subunit structure of soybean 11S globulin [J].Agricultural and Biological Chemistry,1976,40(9):1837-1844.
[3] Utsumi S,Inaba H,Mori T.Heterogeneity of soybean glycinin[J].Phytochemistry,1981,20:585-589.
[4] Badley R A,Atkinson D,Hauser H,et al.The structure,physical and chemical properties of the soybean protein glycinin [J].Biochemistry Biophysics Acta,1975,41:224-228.
[5]Thanh V H,Shibasaki K.Major proteins of soybean seed.Subunit structure of β-conglycinin [J].Journal of Agricultural and Food Chemistry,1978,26:692-295.
[6] Yao J J,Tanteeratarm K,Wei L S.Effects of maturation and storage on solubility,emulsion stability and gelation properties of isolated soy proteins [J].Journal of the American Oil Chemists’Society,1990,67(12):974-979.
[7] Tezuka M,Yagasaki K,Ono T.Changes in characters of soybean glycinin groups Ⅰ,Ⅱa,and Ⅱb caused by heating[J].Journal of Agricultural and Food Chemistry,2004,52:1693-1699.
[8]Utsumi S,Kinsella J E.Force involve in soy protein gelation effect of various reagents on the formation,hardness and solubility of heat-induced made from 7S,11S and soy isolate[J].Journal of Food Science,1985,50:1278-1282.
[9] Hashizume K,Nakamura N,Watanate T.Influence of ionic strength on conformation changes of soybean proteins caused by heating,and relationship of its conformation changes to gel formation[J].Agricultural and Biological Chemistry,1975,39:1339-1347. [10] Fukushima D.Structures of plant storage proteins and their functions [J].Food Reviews International,1991,7(3):353-379.
[11] Aoki H,Taneyama O,Inami M.Emulsifying properties of soy protein:Characteristics of 7S and 11S proteins [J].Jouranl of Food Science,1980,445(3):534-538.
[12] Nir I,Feldman Y,Aserin A,et al.Surface properties and emulsification behavior of denatured soy proteins [J].Journal of Food Science,1994,59:606-609.
[13] Wolf W J,Babcock G E,Smith A K.Purification and stability studies of the 11S component of soybean proteins [J].Archives of Biochemistry and Biophysics,1962,99:265-274.
[14]Thanh V H,Okubo K,Shibasaki K.Isolation and characterization of the multiple 7S globulins of soybean [J].Plant Physiology,1975,56:19-22.
[15] Thanh V H,Okubo K,Shibasaki K.Major proteins of soybean seeds a straight forward fraction and their characterization heterogeneity of beta-conglycinin[J].Journal of Agricultural and Food Chemistry,1976,24:1117-1121.
[16]Nagano T,Hirotsuka M,Mori H,et al.Dynamic viscoelastic study on the gelation of 7S globulin from soybeans [J].Journal of Agricultural and Food Chemistry,1992,40:941-944.
[17] Wu S,Murphy P A,Johnson L A,et al.Simplified process for soybean glycinin and β-conglycinin fractionation[J].Journal of Agricultural and Food Chemistry,2000,48:2702-2708.
[18]周俊清,林亲录.微生物源凝乳酶的研究进展[J].中国食品添加剂,2004,2:6-9.(Zhou J Q,Lin Q L.Studies on the production of chymosin by microorganisms [J].China Food Additives,2004,2:6-9.)
[19] Laemmli U K.Cleavage of structural proteins during the assembly of the head of bacteriophage T4 [J].Nature,1970,227:680-685.
[20] Wolf W J.Physical and chemical properties of soybean proteins[J].Journal of the American Oil Chemists’ Society,1997,54(2):112-117.
[21]韩雅君.大豆分离蛋白的组分分离技术研究[D].北京:中国农业大学,2006.(Han Y J.The study of industrial technology of preparing 7S-rich and 11S-rich soybean protein isolate [D].Beijing:China Agricultural University,2006.)
[22]碱性蛋白酶Alcalase凝固豆浆机理的研究[D].北京:中国农业大学,2005.( Luan G Z.Studies on the mechanism of soymilk coagulation by an alkaline proteinase-alcalase [D].Beijing:China Agricultural University,2005.)

Memo

Memo:
-
Last Update: 2014-10-07