|Table of Contents|

Mapping Quantitative Trait Loci Underlining Tolerance to Phytophthora Root Rot in Soybean(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2008年04期
Page:
572-575
Research Field:
Publishing date:

Info

Title:
Mapping Quantitative Trait Loci Underlining Tolerance to Phytophthora Root Rot in Soybean
Author(s):
LI Xiu-ping1; HAN Ying-peng1; DING Jun-jie2; ZHANG Shu-zhen1; MA Wen-dong3; LI Wen-bin1
1Soybean Research Institute，Northeast Agricultural University，Harbin 150030;
2 Jiamusi Branch of Heilongjiang Academy of Agricultural Sciences；
3 Rice Research Institute of Heilongjiang Academy of Agricultural Sciences；Jiamusi 154026，Heilongjiang，China
Keywords:
Phytophthora root rot; QTLs; Tolerance
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.04.0572
Abstract:
Phytophthora root rot(PRR) caused by Phytophthora sojae M.J.Kaufmann & J.W.Gerdemann is a serious disease of soybeaan world wide.The objective of this study was to identify the location of quantitative trait loci(QTL)in ‘Conrad’,a soybean cultivar with broad tolerance to many races of P.sojae,and ‘Hefeng 25’,a soybean cultivar tolerant to P.sojae in China.The 140 individual of F2：5?RIL population derived from Conrad×Hefeng 25 were adopted，and field and green hourse identification were conducted at Woodslee，Canada and Jiamusi，China in 2007 year，respectively.470 pair of SSR markers were used and 88 of them showed polymorphism.The genetic map was constructed with Mapmaker/EXP3.0b and QTLs analysis was done by CIM method of WinQTL 2.0.Four markers Satt428，Satt600，Satt325,Satt233，from three linkage group MLG D1b+W，MLG Ｆ and MLG Ａ2,were significantly associated with PRR.Each marker explained 5.47% to 27.89% of phenotypic variance.Satt428 and Satt600 were mapped to MLG D1b+W，the genetic distance was 10.9 cM；Satt325 and Satt233 were mapped to MLG F and MLG A2 respectively.Quantitative trait loci on the linkage groups which are associated with PRR was discovered firstly from Heifeng 25，and was mapped to MLG A2.The identified QTLs would be beneficial for marker assistant selection of PRR tolerance varieties against China P.sojae races.

References:

[1] Wrather J A，Anderson T R，Arsyad D M,et al.Soybean disease loss estimates for the top ten soybean producing countries in 1998[J].Canadian Journal of Plant Pathology，2001,23:115-121.

[2]朱振东,王晓鸣,田玉兰,等.防治大豆疫霉根腐病的药剂筛选[J].农药学学报,1999(1):39-44.(Zhu Z D，Wang X M,Tian Y L,et al.Screening of fungicides for controlling phytophthora root rot of soybean[J].Chinese Journal of Pestecide Science，1999(1):39-44.)
[3]沈崇尧,苏彦纯.中国大豆疫霉根腐病的发现及初步研究[J].植物病理学报，1991,21(4):298.(Shen C Y，Su Y C.Discovery and preliminary studies of phytophthora megaspesperma on soybean in China[J].Acta Phytopathologica Sinica,1991,21(4):298.)
[4]Bumham K D，Dorrance A E，Francis D M,et al.Rps 8，a new locus in soybean for resistance to Phytophthora sojae[J].Crop Science,2003,43:101-105.
[5]Gordon S G，St.Martin S K，Dorrance A E.Rps8 maps to a resistance gene rich region on soybean molecular linkage Group F[J].Crop Science,2006,46:168-173.
[6]朱振东,霍云龙,王晓鸣,等.一个抗大豆疫霉根腐病新基因的分子鉴定[J].作物学报，2007,33(1):154-157.(Zhu Z D，Huo Y L,Wang X M，et al.Molecular identification of a novel Phytophthora resistance gene in soybean[J].ActaAgronomica Sinica,2007,33(1):154-157.)
[7]Burnham K D，Francis D，Dorrance M,et al.Genetic Diversity patterns among phytophthora resistant soybean plant introductions based on SSR markers[J].Crop Science,2002,42:338-343.
[8]韩英鹏,李文滨,Terry R.Anderson,等.耐大豆疫霉根腐病QTL定位的研究[J].大豆科学，2006,25(1):23-27.(Han Y P,Li W B,Anderson T R，et al，Mapping quantitative trait loci influencing tolerance to phytophthora root rot in soybean[J].Soybean Science，2006，25（1）：23-27.)
[9]Yu K，Park S J，Poysa V.Abundance and variation of microsallite DNA sequences in beans(Phaseolus and Vigna)[J].Genome,1999，42:27-34.
[10]Bassam B J，Caetano-Anolles G，Gresshoff P M.Fast and sensitive silver staining of DNA in polyacrylamide gels[J].Analytical Biochemistry,1991,196:80-83.
[11]Trigiano R N，Caetano-Anolles G.Laboratory exercises on DNA amplification fingerprinting for evaluating the molecular diversity of horticultural species [J].Hort Technology，1998，8:413-423.
[12]Lander E S，Abrahamson G J，Barlow A.MapMaker: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations[J].Genomics,1987,1:174-181.
[13]Burnham K D，Dorrance A E，VanToai TT，et al.Quantitative trait loci for partial resistance to phytophthora sojae in soybean[J].Crop Science,2003,43:1610-1617.
[14]Diers B W，Mansur L，Imsande J,et al.Mapping phytophthora resistance loci in soybean with restriction fragment length polymorphism markers[J].Crop Science,1992,41:1220-1227.

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Last Update: 2014-10-07