|Table of Contents|

Cloning and Sequence Analysis of an ω-3 Fatty Acid Desaturase Gene from High-oil and Low-oil Soybean Cultivars(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2008年04期
Page:
569-571
Research Field:
Publishing date:

Info

Title:
Cloning and Sequence Analysis of an ω-3 Fatty Acid Desaturase Gene from High-oil and Low-oil Soybean Cultivars
Author(s):
LIU Wen-hao1; LI Wei2; CHEN Xiang-yan3; DAI Hai-ying2; Zhang Li-feng2
1Dairy Cow Research Centre，Shandong Academy of Agriculture Science，Jinan 250100；
2Crop Institute,Shandong Academy of Agriculture Science，Jinan 250100；
3Shandong Academy of Agriculture Science，Jinan 250100，Shandong，China
Keywords:
Soybean; Linolenic acid; ω-3 fatty acid desaturase; Sequence difference
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.04.0569
Abstract:
α-linolenic acid(ALA)is an essential fatty acid which has a lot of healthcare functions to human.In plants，ω-3 fatty acid desaturases(FAD)catalyze linoleic acid(LA)to form ALA.The seed oil of soybean contains high level of ALA.RT-PCR was used to amplify the cDNA of GmFAD3C from the cultivars of Ludou11 and Zheng 9525.The length of the acquired cDNA，containing 380 amino acids，was 1174bp.Nineteen different amino acids were detected in the amino acid sequences of GmFAD3C from Ludou11 and Zheng 9525.These differences will help us to research the differential expression of GmFAD3C between high-oil and low-oil soybean.

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Last Update: 2014-10-07