|Table of Contents|

Use Recombiant PCR to Construct RNAi Expression Vector of Soyabean Lipoxygenase(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2008年04期
Page:
564-568
Research Field:
Publishing date:

Info

Title:
Use Recombiant PCR to Construct RNAi Expression Vector of Soyabean Lipoxygenase
Author(s):
MA Jian; LI Zhi; LIU Yi-ling; WANG Pi-wu
Biology Center of Jilin Agricultural University，Changchun 130118，Jilin，China
Keywords:
Recombinant PCR; RNAi; Lipoxygenase; Expression vector
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.04.0564
Abstract:
For the past few years RNAi was a newly rising territory in biotechnology.RNAi was believed to have huge potential in quality improvement of crops，and it had been successfully applied in quality improvement of rice，wheat and maize，while its application in soybean quality improvement was less documented.During the research of RNAi in plant，RNAi expression vector is always be used，and the commonly used method is time and labor consuming.Recombinant PCR is a high performance method of gene splicing by overlapping.In this research，we use recombinant PCR to construct RNAi expression vector of soybean lipoxygenase，in order to find a easier，faster and generally way to construct RNAi expression vector for soybean.Results showed that constructing RNAi expression vector of the lipoxygenase gene by recombinant PCR technology is feasible，and the method is more rapid，simple and convenient than common methods.

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Last Update: 2014-10-07