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Construction and Analysis of A SSH Library of Soybean Upon Infection with Phytophthora sojae(PDF)

《大豆科学》[ISSN:1000-9841/CN:23-1227/S]

Issue:
2008年03期
Page:
543-545
Research Field:
Publishing date:

Info

Title:
Construction and Analysis of A SSH Library of Soybean Upon Infection with Phytophthora sojae
Author(s):
ZHANG Shu-zhen1; XU Peng-fei1; WU Jun-jiang2; LI Wen-bin1; CHEN Wei-yuan3; CHEN Chen1; YU An-liang1; WANG Jin-sheng1; JIN Li-mei1; LI Cen1
1Soybean Research InstitutNortheastAgricultural University;Chinese Education Ministry’s Key Laboratory of Soybean Biology，Harbin 150030;
2 Soybean Research Institute，Heilongjiang Academy of Agricultural Sciences (HAAS)，Harbin 150086;
3 Suihua Research Institute of HAAS,Suihua 152052，Heilongjiang，China
Keywords:
Phytophthora root rot of soybean; Suppression subtractive hybridization(SSH) ; cDNA library
PACS:
S565.1
DOI:
10.11861/j.issn.1000-9841.2008.03.0543
Abstract:
Phytophthora root and stem rot of soybean(Phytophthora sojae) is one of destructive diseases endangering soybean production，but there have been few reports on gene expression after inoculated with pathogen.In this study ，SSH cDNA library of resistance soybean variety Suinong 10 inoculated with race 1 of P.sojae was constructed successfully，and 2 067 positive clones were screened.The length of most of the insert fragments was focused on 100-800 bp.375 functional ESTs have been gotten，and they dealt with the effect of inhibition pathogen growth，cell self-protection，signal transduction，system acquired resistance，protein synthesis and respiration，etc.after compared with sequences in unigene database of GeneBank with BLASTn and BLASTx algorithm.

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Last Update: 2014-10-08